Biomimetic microspheres for 3D mesenchymal stem cell culture and characterization

Autor: Amparo Sempere, Sandra Clara-Trujillo, Juan Carlos Marín-Payá, Gloria Gallego Ferrer, Lourdes Cordón, José Luis Gómez Ribelles
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Surface Properties
Swine
3D environment
02 engineering and technology
01 natural sciences
Polymerization
Extracellular matrix
Extracellular matrix coatings
Paracrine signalling
chemistry.chemical_compound
Colloid and Surface Chemistry
Surface modification
Biomimetic Materials
0103 physical sciences
Hyaluronic acid
Cell Adhesion
Animals
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
Physical and Theoretical Chemistry
Particle Size
Cell adhesion
Cells
Cultured
Cell Proliferation
010304 chemical physics
biology
Molecular Structure
Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
Surfaces and Interfaces
General Medicine
021001 nanoscience & nanotechnology
Microspheres
Cell biology
Fibronectin
Cell culture
MAQUINAS Y MOTORES TERMICOS
biology.protein
Mesenchymal stem cells
Stem cell
0210 nano-technology
TERMODINAMICA APLICADA (UPV)
Biotechnology
Zdroj: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
COLLOIDS AND SURFACES B-BIOINTERFACES
r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe
ISSN: 0927-7765
Popis: [EN] Stem cells reside in niches, specialized microenvironments that sustain and regulate their fate. Extracellular matrix (ECM), paracrine factors or other cells are key niche regulating elements. As the conventional 2D cell culture lacks these elements, it can alter the properties of naive stem cells. In this work we designed a novel biomimetic microenvironment for cell culture, consisting of magnetic microspheres, prepared with acrylates and acrylic acid copolymers and functionalized with fibronectin or hyaluronic acid as ECM coatings. To characterize cell proliferation and adhesion, porcine mesenchymal stem cells (MSCs) were grown with the different microspheres. The results showed that the 3D environments presented similar proliferation to the 2D culture and that fibronectin allows cell adhesion, while hyaluronic acid hinders it. In the 3D environments, cells reorganize the microspheres to grow in aggregates, highlighting the advantages of microspheres as 3D environments and allowing the cells to adapt the environment to their requirements.
PROMETEO/2016/063 project is acknowledged. This work was partially financed with FEDER funds (CIBERONC (CB16/12/00284)). The CIBER-BBN initiative is funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. This work was also supported by the Spanish Ministry of Science, Innovation and Universities trough Sandra Clara-Trujillo FPU17/05810 grant.
Databáze: OpenAIRE
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