Ii-Key/MHC class II epitope hybrid peptide vaccines for HIV
| Autor: | Nikoletta L. Kallinteris, Joseph V Gulfo, Yu Li, Minzhen Xu, Shuzhen Wu, Robert E. Humphreys, Hongliang Hu, Xueqing Lu |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
CD74
T cell Genes MHC Class II HIV Core Protein p24 Epitope HIV Envelope Protein gp160 Epitopes Mice Antigen MHC class I medicine Animals AIDS Vaccines Mice Inbred C3H MHC class II General Veterinary General Immunology and Microbiology biology ELISPOT Histocompatibility Antigens Class II Public Health Environmental and Occupational Health MHC restriction Virology Molecular biology Antigens Differentiation B-Lymphocyte Infectious Diseases medicine.anatomical_structure Vaccines Subunit biology.protein Molecular Medicine Immunization Spleen |
| Zdroj: | Vaccine. 21:4128-4132 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(03)00493-6 |
| Popis: | The Ii-Key/MHC class II epitope hybrid acts on MHC class II molecules to facilitate replacement of antigenic peptides with the epitope tethered to the Ii-Key motif. Hybrid peptides linking an immunoregulatory segment of the Ii protein (Ii-Key peptide) through a polymethylene bridge to MHC class II epitopes of HIV gp160 or gag are potent vaccines to elicit CD4(+) T cell responses. More potent responses to two MHC class II epitopes, HIV gp160(843-852) or HIV gag(279-292), occurred in mice immunized with Ii-Key hybrid peptides than with epitope-only peptides, as measured in IL-4 and IFN-gamma ELISPOT assays of splenic lymphocytes stimulated in vitro by epitope-only peptides. Both the number of responding cells and cytokine output per cell were increased. The Ii-Key/MHC class II epitope hybrid acts on MHC class II molecules to facilitate replacement of antigenic peptides with the epitope tethered to the Ii-Key motif. Such antigenic peptide constructs create opportunities to enhance greatly Th1 or Th2 responses to MHC class II epitopes for therapeutic purposes. |
| Databáze: | OpenAIRE |
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