Design of Light-Controlled Protein Conformations and Functions
| Autor: | Ryan Ritterson, Tanja Kortemme, Kyle A. Barlow, Daniel Hoersch |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Light Chemistry Protein Conformation Protein design Protein engineering 010402 general chemistry 01 natural sciences Small molecule Mass Spectrometry Article 0104 chemical sciences Chaperonin 03 medical and health sciences 030104 developmental biology Protein structure Searching the conformational space for docking Nat Mutation Electrophoresis Polyacrylamide Gel Spectrophotometry Ultraviolet Biological system Protocol (object-oriented programming) |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493935673 |
| ISSN: | 1940-6029 |
| Popis: | In recent years, interest in controlling protein function with light has increased. Light offers a number of unique advantages over other methods, including spatial and temporal control and high selectivity. Here, we describe a general protocol for engineering a protein to be controllable with light via reaction with an exogenously introduced photoisomerizable small molecule and illustrate our protocol with two examples from the literature: the engineering of the calcium affinity of the cell-cell adhesion protein cadherin, which is an example of a protein that switches from a native to a disrupted state (Ritterson et al. J Am Chem Soc (2013) 135:12516-12519), and the engineering of the opening and closing of the chaperonin Mm-cpn, an example of a switch between two functional states (Hoersch et al.: Nat Nanotechn (2013) 8:928-932). This protocol guides the user from considering which proteins may be most amenable to this type of engineering, to considerations of how and where to make the desired changes, to the assays required to test for functionality. |
| Databáze: | OpenAIRE |
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