pH-Sensitive photoinduced energy transfer from bacteriorhodopsin to single-walled carbon nanotubes in SWNT-bR hybrids
| Autor: | Patricia Bertoncini, Karim El Hadj, Olivier Chauvet |
|---|---|
| Přispěvatelé: | Institut des Matériaux Jean Rouxel (IMN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN) |
| Rok vydání: | 2013 |
| Předmět: |
Photoluminescence
Materials science General Physics and Astronomy 02 engineering and technology Carbon nanotube 010402 general chemistry Photochemistry Microscopy Atomic Force 7. Clean energy 01 natural sciences law.invention law General Materials Science Photoluminescence excitation single-walled carbon nanotubes . bacteriorhodopsin . biohybrids . fluorescence . energy transfer Aqueous solution biology Nanotubes Carbon General Engineering Bacteriorhodopsin Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Optical properties of carbon nanotubes Spectrometry Fluorescence Energy Transfer Bacteriorhodopsins [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci] biology.protein Spectrophotometry Ultraviolet Absorption (chemistry) 0210 nano-technology |
| Zdroj: | ACS Nano ACS Nano, American Chemical Society, 2013, 7 (10), pp.8743. ⟨10.1021/nn403092r⟩ |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/nn403092r⟩ |
| Popis: | International audience; Energy transfer mechanisms in noncovalently bound bacteriorhodopsin/single-walled carbon nanotube (SWNT) hybrids are investigated using optical absorption and photoluminescence excitation measurements. The morphology of the hybrids was investigated by atomic force microscopy. In this study, proteins are immobilized onto the sidewall of the carbon nanotubes using a sodium cholate suspension-dialysis method that maintains the intrinsic optical and fluorescence properties of both molecules. The hybrids are stable in aqueous solutions for pH ranging from 4.2 to 9 and exhibit photoluminescence properties that are pH-dependent. The study reveals that energy transfer from bacteriorhodopsin to carbon nanotubes takes place. So, at pH higher than 5 and up to 9, the SWNTs absorb the photons emitted by the aromatic residues of the protein, inducing a strong increase in intensity of the E11 emissions of SWNTs through their E33 and E44 excitations. From pH = 4.2 to pH = 5, the protein fluorescence is strongly quenched whatever the emission wavelengths, while additional fluorescence features appear at excitation wavelengths ranging from 660 to 680 nm and at 330 nm. The presence of these features is attributed to a resonance energy transfer mechanism that has an efficiency of 0.94 ± 0.02. More, by increasing the pH of the dispersion, the fluorescence characteristics become those observed at higher pH values and vice versa. |
| Databáze: | OpenAIRE |
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