Hydrogen Indirectly Suppresses Increases in Hydrogen Peroxide in Cytoplasmic Hydroxyl Radical-Induced Cells and Suppresses Cellular Senescence

Autor: Takahiro Sakai, Hirano Shinichi, Ryosuke Kurokawa, Jun Imai
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Cytoplasm
lipid peroxide
Eukaryotic Initiation Factor-2
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
fluids and secretions
cellular senescence
Hydrogen peroxide
lcsh:QH301-705.5
Spectroscopy
Lipid peroxide
cytoplasmic hydroxyl radical
Chemistry
Kinase
digestive
oral
and skin physiology
General Medicine
Glutathione
Computer Science Applications
Cell biology
Phosphorylation
Intracellular
Signal Transduction
Cyclin-Dependent Kinase Inhibitor p21
hydrogen peroxide
digestive system
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Humans
Physical and Theoretical Chemistry
Molecular Biology
Cyclin-Dependent Kinase Inhibitor p16
Hydroxyl Radical
Organic Chemistry
Fibroblasts
Activating Transcription Factor 4
Oxidative Stress
030104 developmental biology
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
Cell culture
hydrogen
Hydroxyl radical
Lipid Peroxidation
030217 neurology & neurosurgery
DNA Damage
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 2, p 456 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 2
ISSN: 1422-0067
Popis: Bacteria inhabiting the human gut metabolize microbiota-accessible carbohydrates (MAC) contained in plant fibers and subsequently release metabolic products. Gut bacteria produce hydrogen (H2), which scavenges the hydroxyl radical (&bull
OH). Because H2 diffuses within the cell, it is hypothesized that H2 scavenges cytoplasmic &bull
OH (cyto &bull
OH) and suppresses cellular senescence. However, the mechanisms of cyto &bull
OH-induced cellular senescence and the physiological role of gut bacteria-secreted H2 have not been elucidated. Based on the pyocyanin-stimulated cyto &bull
OH-induced cellular senescence model, the mechanism by which cyto &bull
OH causes cellular senescence was investigated by adding a supersaturated concentration of H2 into the cell culture medium. Cyto &bull
OH-generated lipid peroxide caused glutathione (GSH) and heme shortage, increased hydrogen peroxide (H2O2), and induced cellular senescence via the phosphorylation of ataxia telangiectasia mutated kinase serine 1981 (p-ATMser1981)/p53 serine 15 (p-p53ser15)/p21 and phosphorylation of heme-regulated inhibitor (p-HRI)/phospho-eukaryotic translation initiation factor 2 subunit alpha serine 51 (p-eIF2&alpha
)/activating transcription factor 4 (ATF4)/p16 pathways. Further, H2 suppressed increased H2O2 by suppressing cyto &bull
OH-mediated lipid peroxide formation and cellular senescence induction via two pathways. H2 produced by gut bacteria diffuses throughout the body to scavenge cyto &bull
OH in cells. Therefore, it is highly likely that gut bacteria-produced H2 is involved in intracellular maintenance of the redox state, thereby suppressing cellular senescence and individual aging. Hence, H2 produced by intestinal bacteria may be involved in the suppression of aging.
Databáze: OpenAIRE
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