The horizontal transfer of Pseudomonas aeruginosa PA14 ICE PAPI-1 is controlled by a transcriptional triad between TprA, NdpA2 and MvaT
| Autor: | Moly Ba, Victoria Schmidt, Sophie de Bentzmann, Gaël Chambonnier, Corinne Sebban-Kreuzer, Christophe Bordi, Gauthier Dangla-Pélissier, Caroline Giraud, Nicolas Roux |
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| Přispěvatelé: | Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU), Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Dynamique cellulaire et moléculaire de la muqueuse respiratoire, Université de Reims Champagne-Ardenne (URCA)-IFR53-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Recherche Risques Microbiens (U2RM), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Schmidt, Victoria |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
MESH: Chromosomes
Bacterial Gene Transfer Horizontal Genomic Islands Transcription Genetic AcademicSubjects/SCI00010 Virulence Factors MESH: Trans-Activators Virulence MESH: Biofilms Biology medicine.disease_cause Genome Regulon MESH: Gene Expression Profiling Bacterial Proteins [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Genetics medicine MESH: Genomic Islands Gene MESH: Bacterial Proteins MESH: Virulence Factors MESH: Gene Expression Regulation Bacterial Pseudomonas aeruginosa Gene Expression Profiling MESH: Transcription Genetic Gene regulation Chromatin and Epigenetics Biofilm MESH: Conjugation Genetic Gene Expression Regulation Bacterial Chromosomes Bacterial Pathogenicity island [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Gene Transfer Horizontal MESH: DNA Transposable Elements Biofilms Conjugation Genetic Horizontal gene transfer MESH: Pseudomonas aeruginosa MESH: Regulon DNA Transposable Elements Trans-Activators [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, 2021, 49 (19), pp.10956-10974. ⟨10.1093/nar/gkab827⟩ |
| ISSN: | 0305-1048 1362-4962 |
| DOI: | 10.1093/nar/gkab827⟩ |
| Popis: | Pseudomonas aeruginosa is a major cause of nosocomial infections, particularly in immunocompromised patients or in individuals with cystic fibrosis. Genome sequences reveal that most P. aeruginosa strains contain a significant number of accessory genes gathered in genomic islands. Those genes are essential for P. aeruginosa to invade new ecological niches with high levels of antibiotic usage, like hospitals, or to survive during host infection by providing pathogenicity determinants. P. aeruginosa pathogenicity island 1 (PAPI-1), one of the largest genomic islands, encodes several putative virulence factors, including toxins, biofilm genes and antibiotic-resistance traits. The integrative and conjugative element (ICE) PAPI-1 is horizontally transferable by conjugation via a specialized GI-T4SS, but the mechanism regulating this transfer is currently unknown. Here, we show that this GI-T4SS conjugative machinery is directly induced by TprA, a regulator encoded within PAPI-1. Our data indicate that the nucleotide associated protein NdpA2 acts in synergy with TprA, removing a repressive mechanism exerted by MvaT. In addition, using a transcriptomic approach, we unravelled the regulon controlled by Ndpa2/TprA and showed that they act as major regulators on the genes belonging to PAPI-1. Moreover, TprA and NdpA2 trigger an atypical biofilm structure and enhance ICE PAPI-1 transfer. |
| Databáze: | OpenAIRE |
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