Physically Cross-linked Hydrogels of β -cyclodextrin Polymer and Poly(ethylene glycol)-cholesterol as Delivery Systems for Macromolecules and Small Drug Molecules
| Autor: | Shaaban K. Osman, Ghareb M. Soliman, Saleh Abd El Rasoul |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Biocompatibility
Cell Survival Surface Properties Chemistry Pharmaceutical Pharmaceutical Science macromolecular substances complex mixtures Polyethylene Glycols Mice chemistry.chemical_compound Differential scanning calorimetry X-Ray Diffraction Polymer chemistry Animals Technology Pharmaceutical Molecule Cytotoxicity Drug Carriers Riluzole Calorimetry Differential Scanning beta-Cyclodextrins Temperature technology industry and agriculture Hydrogels Elasticity Kinetics Cholesterol Solubility Polymerization chemistry Immunoglobulin G Self-healing hydrogels Microscopy Electron Scanning NIH 3T3 Cells Biophysics lipids (amino acids peptides and proteins) Rheology Ethylene glycol Powder Diffraction Macromolecule |
| Zdroj: | Current Drug Delivery. 12:415-424 |
| ISSN: | 1567-2018 |
| DOI: | 10.2174/1567201812666150326113331 |
| Popis: | An injectable hydrogel based on the inclusion complexation of polymerized β-cyclodextrin (pβ-CD) and cholesterol terminated poly(ethylene glycol) (PEG-chol) was developed and used as a delivery system for both macromolecules and small drugs. The hydrogel was characterized by different analyses including X-ray diffraction, differential scanning calorimetry and scanning electron microscopy. The effects of pβ-CD/PEG-chol ratio and PEG-chol architecture on the hydrogel properties were also investigated. Cytotoxicity of the hydrogel was evaluated in NIH 3T3 fibroblasts using MTS assay. The hydrogel had an elastic behavior even at high temperature since the gelation temperature was observed at 68 °C. The highest hydrogel strength and stability were observed for the 8-armed PEG-chol at a pβ-CD/PEG-chol ratio of 1:1, w/w. Hydrogel degradation in phosphate buffered saline occurred by gradual erosion over the course of two months. IgG, a model hydrophilic macromolecule and riluzole, a model hydrophobic small drug were incorporated into the hydrogel and quantitatively released in a sustained fashion. The released IgG maintained its bioactivity confirming the absence of deleterious effects on protein structure during loading and release. The hydrogels showed no toxicity on NIH 3T3 fibroblasts confirming their biocompatibility. These results confirm the potential of pβ-CD/PEG-chol hydrogel as a versatile delivery system for drugs of different molecular weights and nature. |
| Databáze: | OpenAIRE |
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