Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture
| Autor: | Adamczyk-Sowa M, Polaniak R, Stanislaw J. Konturek, E. Birkner, Drzazga Z, Paweł Sowa, Tomasz Brzozowski, Zwirska-Korczala K |
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| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Time Factors Extremely low frequency magnetic field Endocrinology Diabetes and Metabolism Clinical Biochemistry Uterine Cervical Neoplasms Biochemistry Antioxidants Inorganic Chemistry Lipid peroxidation Melatonin Superoxide dismutase chemistry.chemical_compound Mice Electromagnetic Fields Internal medicine Cell Line Tumor Malondialdehyde medicine Animals Basal cell chemistry.chemical_classification Glutathione Peroxidase biology Superoxide Dismutase Glutathione peroxidase Biochemistry (medical) General Medicine respiratory system Molecular biology Enzyme assay Oxidative Stress Endocrinology chemistry biology.protein Carcinoma Squamous Cell Female medicine.drug |
| Zdroj: | Biological trace element research. 102(1-3) |
| ISSN: | 0163-4984 |
| Popis: | Effects of melatonin, extremely-low-frequency magnetic field (ELF-MF), and their combination on AT478 murine squamous cell carcinoma line were studied. Manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (Cu/ZnSOD), and glutathione peroxidase (GSH-Px) were used as markers of cells antioxidative status, and malondialdehyde (MDA) level was used as a marker of lipid peroxidation. After melatonin treatment, antioxidative enzyme activities were increased and MDA level was decreased. Application of ELF-MF on treated cells caused an increase of both superoxide dismutases activity and MDA level, but influence of ELF-MF on GSH-Px activity was negligible. All enzyme activity in culture medium containing melatonin (10−3, 10−4, 10−5 M) after exposure to ELF-MF were significantly diminished compared to cells treated only with melatonin. Also MDA levels after combined treatment with melatonin and ELF-MF were significantly decreased. Observed changes were statistically significant (p |
| Databáze: | OpenAIRE |
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