The Gut of Healthy Infants in the Community as a Reservoir of ESBL and Carbapenemase-Producing Bacteria
| Autor: | Patrick C. Seed, Ahreen Allana, Cristina A Salinas, Mehreen Arshad, Asia Khan, Shahida Qureshi, Lauren Hale, Najeeb Rahman, Raul E. Salinas, Aneeta Hotwani, Alondra Diaz, Anita Kaniz Mehdi Zaidi, Ali Faisal Saleem |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
multi-drug resistant extended-spectrum beta-lactamase medicine.disease_cause Biochemistry Microbiology Article carbapenemase Enterobacteriaceae Multiplex polymerase chain reaction gut colonization medicine Pharmacology (medical) Colonization Typing General Pharmacology Toxicology and Pharmaceutics Escherichia coli Phylogenetic tree biology infants lcsh:RM1-950 Antimicrobial biology.organism_classification Infectious Diseases lcsh:Therapeutics. Pharmacology Bacteria |
| Zdroj: | Antibiotics Volume 9 Issue 6 Antibiotics, Vol 9, Iss 286, p 286 (2020) |
| ISSN: | 2079-6382 |
| DOI: | 10.3390/antibiotics9060286 |
| Popis: | The recent rapid rise of multi-drug resistant Enterobacteriaceae (MDR-E) is threatening the treatment of common infectious diseases. Infections with such strains lead to increased mortality and morbidity. Using a cross-sectional study, we aimed to estimate the prevalence of gut colonization with extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae among healthy infants born in Pakistan, a setting with high incidence of MDR-E infections. Stool samples were collected from 104 healthy infants between the ages of 5 and 7 months. Enterobacteriaceae isolates were screened for resistance against several antimicrobial classes. Presence of ESBL and carbapenemase genes was determined using multiplex PCR. Sequence types were assigned to individual strains by multi-locus sequence typing. Phylogenetic analysis of Escherichia coli was done using the triplex PCR method. Forty-three percent of the infants were positive for ESBL-producing Enterobacteriaceae, the majority of which were E. coli. We identified several different ESBL E. coli sequence types most of which belonged to the phylogenetic group B2 (23%) or D (73%). The widespread colonization of infants in a developing country with ESBL-producing Enterobacteriaceae is concerning. The multiple sequence types and reported non-human sources support that multiple non-epidemic MDR lineages are circulating in Pakistan with healthy infants as a common reservoir. |
| Databáze: | OpenAIRE |
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