Effect of methotrexate on cerebellar development in infant rats
| Autor: | Kota Ueda, Akihiko Sugiyama, Jing Sun, Satoshi Furukawa, Takashi Takeuchi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
cerebellar hypoplasia medicine.medical_specialty Cerebellum external granular layer Granular layer External Granular Layer methotrexate Internal medicine mental disorders Pathology In Situ Nick-End Labeling medicine Animals rat Rats Wistar inhibition of cell proliferation TUNEL assay Full Paper General Veterinary Chemistry Granule (cell biology) apoptosis Organ Size Rats Endocrinology medicine.anatomical_structure Animals Newborn nervous system Apoptosis Folic Acid Antagonists Female Methotrexate After treatment medicine.drug |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Six-day-old rats were treated intraperitoneal injections with methotrexate 1 mg/kg, and the cerebellum was examined. Both the length and width of the vermis decreased in the methotrexate-treated group instead of the control from 4 day after treatment (DAT) onward. A significant reduction in the width of the external granular layer was detected on 2 and 3 DAT in the methotrexate group. By 4 DAT, the width of the external granular layer of the methotrexate group was indistinguishable from the control, and by 8 DAT, it was greater than that of the control. The molecular layer of methotrexate group on 8 and 15 DAT was thinner than that of the control. On 1 DAT, in the methotrexate group, there were many TUNEL and cleaved caspase-3-positive granular cells throughout the external granular layer, and they decreased time-dependently. On 1 DAT, in the methotrexate group, phospho-histone H3-positive cells in the external granular layer were fewer than in the control and tended to increase on 2-4 DAT. The p21-positive-rate of the external granule cells in the MTX group was higher than in the control on 1-4 DAT. These results suggested that methotrexate exposure on postnatal day 6 induces a delay, slowing in the migration of external granular cells to the inner granular layer, attributed to decrease or inhibition in the production of external granular cells that had arisen from apoptosis and the decrease in cell proliferative activity, resulting in cerebellar hypoplasia. |
| Databáze: | OpenAIRE |
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