Differentially Expressed MicroRNAs in Meningiomas Grades I and II Suggest Shared Biomarkers with Malignant Tumors
| Autor: | Kristin Smistad Myrmel, Dag H. Coucheron, Anita Ursvik, Mona Nystad, Mari Walquist, Rune Hennig, Morten Andreassen, Mohamed Raafat El-Gewely, Steinar Johansen, Erik Knutsen |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Small RNA Pathology medicine.medical_specialty SOLiD deep sequencing lcsh:RC254-282 meningioma Article CDH1 Meningioma 03 medical and health sciences 0302 clinical medicine microRNA medicine miRNA RT-qPCR cap cells and Immunohistochemistry (IHC) PTEN Messenger RNA biology Mathematics and natural science: 400::Basic biosciences: 470::Cell biology: 471 [VDP] Meninges lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease nervous system diseases VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 [VDP] biology.protein Biomarker (medicine) |
| Zdroj: | Cancers; Volume 8; Issue 3; Pages: 31 Cancers Cancers, Vol 8, Iss 3, p 31 (2016) |
| Popis: | Publisher's version, source http://doi.org/10.3390/cancers8030031 Meningiomas represent the most common primary tumors of the central nervous system, but few microRNA (miRNA) profiling studies have been reported so far. Deep sequencing of small RNA libraries generated from two human meningioma biopsies WHO grades I (benign) and II (atypical) were compared to excess dura controls. Nineteen differentially expressed miRNAs were validated by RT-qPCR using tumor RNA from 15 patients and 5 meninges controls. Tumor suppressor miR-218 and miR-34a were upregulated relative to normal controls, however, miR-143, miR-193b, miR-451 and oncogenic miR-21 were all downregulated. From 10 selected putative mRNA targets tested by RT-qPCR only four were differentially expressed relative to normal controls. PTEN and E-cadherin (CDH1) were upregulated, but RUNX1T1 was downregulated. Proliferation biomarker p63 was upregulated with nuclear localization, but not detected in most normal arachnoid tissues. Immunoreactivity of E-cadherin was detected in the outermost layer of normal arachnoids, but was expressed throughout the tumors. Nuclear Cyclin D1 expression was positive in all studied meningiomas, while its expression in arachnoid was limited to a few trabecular cells. Meningiomas of grades I and II appear to share biomarkers with malignant tumors, but with some additional tumor suppressor biomarkers expression. Validation in more patients is of importance. |
| Databáze: | OpenAIRE |
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