Microtubule end tethering of a processive Kinesin-8 motor Kif18b is required for spindle positioning
| Autor: | Julie P.I. Welburn, Toni McHugh, A. Agata Głuszek |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Kinesins
Mitosis macromolecular substances Spindle Apparatus Microtubules Article 03 medical and health sciences 0302 clinical medicine Microtubule biochemistry Humans Microtubule end Kinesin 8 Metaphase Research Articles 030304 developmental biology Anaphase Physics 0303 health sciences fungi cell cycle and division Spindle apparatus Cell biology biological phenomena cell phenomena and immunity CRISPR-Cas Systems Astral microtubules Microtubule-Associated Proteins 030217 neurology & neurosurgery Cell Division HeLa Cells Protein Binding |
| Zdroj: | The Journal of Cell Biology McHugh, T, Gluszek, A A & Welburn, J P I 2018, ' Microtubule end tethering of a processive kinesin-8 motor Kif18b is required for spindle positioning ', Journal of Cell Biology, vol. 217, no. 7, pp. 2403-2416 . https://doi.org/10.1083/jcb.201705209 |
| DOI: | 10.1101/286658 |
| Popis: | Kinesin-8 Kif18b shortens astral microtubules in mitosis. Combining cell biology and biochemical reconstitution, McHugh et al. show that Kif18b walks and accumulates to microtubule plus ends in a phosphospecific manner to regulate astral microtubule dynamics and center the mitotic spindle. Mitotic spindle positioning specifies the plane of cell division during anaphase. Spindle orientation and positioning are therefore critical to ensure symmetric division in mitosis and asymmetric division during development. The control of astral microtubule length plays an essential role in positioning the spindle. In this study, using gene knockout, we show that the kinesin-8 Kif18b controls microtubule length to center the mitotic spindle at metaphase. Using in vitro reconstitution, we reveal that Kif18b is a highly processive plus end–directed motor that uses a C-terminal nonmotor microtubule-binding region to accumulate at growing microtubule plus ends. This region is regulated by phosphorylation to spatially control Kif18b accumulation at plus ends and is essential for Kif18b-dependent spindle positioning and regulation of microtubule length. Finally, we demonstrate that Kif18b shortens microtubules by increasing the catastrophe rate of dynamic microtubules. Overall, our work reveals that Kif18b uses its motile properties to reach microtubule ends, where it regulates astral microtubule length to ensure spindle centering. |
| Databáze: | OpenAIRE |
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