The Production of Interleukin-11 and Decidualization Are Compromised in Endometrial Stromal Cells Derived from Patients with Infertility
| Autor: | John K. Heath, Jung Hye Hwang, David H. Barlow, Helen J. Mardon, Natalia Karpovich, Petra A.B. Klemmt, J. Enda McVeigh |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
medicine.medical_specialty Stromal cell Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Cell 8-Bromo Cyclic Adenosine Monophosphate Biology Endometrium Biochemistry Article Endocrinology Internal medicine Cyclic AMP Decidua medicine Humans Receptors Interleukin-11 Interleukin-11 Receptor alpha Subunit Cells Cultured Biochemistry (medical) Decidualization Receptors Interleukin Interleukin-11 Prolactin Insulin-Like Growth Factor Binding Protein 1 Interleukin 11 medicine.anatomical_structure Cytokine Female Stromal Cells Infertility Female Signal Transduction |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 90:1607-1612 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jc.2004-0868 |
| Popis: | IL-11 signaling is critical for decidualization of the endometrial stroma in early pregnancy in the mouse. In this study, we investigate the function of IL-11 signaling in cAMP-induced decidualization of human endometrial stromal cells. We show that treatment of endometrial stromal cells with 8-bromo-cAMP (8-Br-cAMP) results in an increase in the levels of secreted IL-11, whereas levels of cell surface IL-11 receptor alpha are similar with or without 8-Br-cAMP treatment. The production of IL-11 correlates with the production of molecular markers of decidualization, prolactin and IGF-binding protein-1. The expression of these markers is inhibited when IL-11 signaling is specifically blocked in decidualizing endometrial stromal cells by the IL-11 antagonist W147A. We demonstrate that 8-Br-cAMP-induced endometrial stromal cells derived from patients with primary infertility produce lower levels of prolactin, IGF-binding protein-1, and IL-11 than cells derived from fertile women. Our results suggest that IL-11 expression is critically important during decidualization in the human endometrium, and that aberrant regulation of endometrial IL-11 production may be associated with some types of infertility. |
| Databáze: | OpenAIRE |
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