AtRAE1 is involved in degradation of ABA receptor RCAR1 and negatively regulates ABA signalling inArabidopsis

Autor: Xufeng Li, Jianmei Wang, Ying Li, Xiaoyu Wang, Xiangge Kong, Xiaoyi Li, Zhibin Liu, Yi Yang, Dekuan Li, Liang Zhang
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Proteasome Endopeptidase Complex
Physiology
Arabidopsis
Plant Science
Pyruvate dehydrogenase phosphatase
Genes
Plant
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Ubiquitin
Gene Expression Regulation
Plant
Stress
Physiological
Two-Hybrid System Techniques
Sense (molecular biology)
Receptor
Abscisic acid
Dehydration
biology
Arabidopsis Proteins
Chemistry
organic chemicals
fungi
Intracellular Signaling Peptides and Proteins
food and beverages
RNA
biology.organism_classification
Adaptation
Physiological
Droughts
Ubiquitin ligase
Nuclear Pore Complex Proteins
030104 developmental biology
Biochemistry
Mutation
Proteolysis
biology.protein
Carrier Proteins
Abscisic Acid
Protein Binding
Signal Transduction
010606 plant biology & botany
Zdroj: Plant, Cell & Environment. 41:231-244
ISSN: 0140-7791
Popis: The phytohormone abscisic acid (ABA) plays an important role in regulating plant growth, development, and adaption to various environmental stresses. Regulatory components of ABA receptors (RCARs, also known as PYR/PYLs) sense ABA and initiate ABA signalling through inhibiting the activities of protein phosphatase 2C in Arabidopsis. However, the way in which ABA receptors are regulated is not well known. A DWD protein AtRAE1 (for RNA export factor 1 in Arabidopsis), which may act as a substrate receptor of CUL4-DDB1 E3 ligase, is an interacting partner of RCAR1/PYL9. The physical interaction between RCAR1 and AtRAE1 is confirmed in vitro and in vivo. Overexpression of AtRAE1 in Arabidopsis causes reduced sensitivity of plants to ABA, whereas suppression of AtRAE1 causes increased sensitivity to ABA. Analysis of protein stability demonstrates that RCAR1 is ubiquitinated and degraded in plant cells and AtRAE1 regulates the degradation speed of RCAR1. Our findings indicate that AtRAE1 likely participates in ABA signalling through regulating the degradation of ABA receptor RCAR1.
Databáze: OpenAIRE
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