Copper-Zinc Superoxide Dismutase (SOD1) Is Released by Microglial Cells and Confers Neuroprotection against 6-OHDA Neurotoxicity
| Autor: | Marco Caprini, Elisabetta Polazzi, Ilaria Mengoni, Barbara Monti, Emiliano Peña-Altamira, Ewelina Kurtys |
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| Přispěvatelé: | Polazzi E., Mengoni I., Caprini M., Peña-Altamira E., Kurtys E., Monti B. |
| Rok vydání: | 2012 |
| Předmět: |
Cerebellar granule neurons
Cell Survival SOD1 Neuroprotection lcsh:RC346-429 Superoxide dismutase Cellular and Molecular Neuroscience Superoxide Dismutase-1 Developmental Neuroscience In vivo medicine Animals Rats Wistar Oxidopamine Conditioned medium Cells Cultured lcsh:Neurology. Diseases of the nervous system Secretory pathway Neurons Dose-Response Relationship Drug biology Microglia Superoxide Dismutase Chemistry lcsh:QP351-495 Dopaminergic Neurotoxicity nutritional and metabolic diseases medicine.disease Rats nervous system diseases Cell biology Neuroprotective Agents lcsh:Neurophysiology and neuropsychology medicine.anatomical_structure Animals Newborn nervous system Neurology biology.protein 6-Hydroxydopamine Neuroscience |
| Zdroj: | Neurosignals, Vol 21, Iss 1-2, Pp 112-128 (2012) |
| ISSN: | 1424-8638 1424-862X |
| DOI: | 10.1159/000337115 |
| Popis: | Microglial-neuronal interactions are essential for brain physiopathology. In this framework, recent data have changed the concept of microglia from essentially macrophagic cells to crucial elements in maintaining neuronal homeostasis and function through the release of neuroprotective molecules. Using proteomic analysis, here we identify copper-zinc superoxide dismutase (SOD1) as a protein produced and released by cultured rat primary microglia. Evidence for a neuroprotective role of microglia-derived SOD1 resulted from experiments in which primary cerebellar granule neurons (CGNs) were exposed to the dopaminergic toxin 6-hydroxydopamine (6-OHDA). Microglial conditioned medium, in which SOD1 had accumulated, protected CGNs from degeneration, and neuroprotection was abrogated by SOD1 inhibitors. These effects were replicated when exogenous SOD1 was added to a nonconditioned medium. SOD1 neuroprotective action was mediated by increased cell calcium from an external source. Further experiments demonstrated the specificity of SOD1 neuroprotection against 6-OHDA compared to other types of neurotoxic challenges. SOD1, constitutively produced and released by microglia through a lysosomal secretory pathway, is identified here for the first time as an essential component of neuroprotection mediated by microglia. This novel information is relevant to stimulating further studies of microglia-mediated neuroprotection in in vivo models of neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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