Strong and specific effects of cations on lysozyme chloride solubility
| Autor: | Madeleine Riès-Kautt, Laurent Legrand, Philippe Benas |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Hofmeister series
Inorganic chemistry Salt (chemistry) Chloride law.invention Crystal chemistry.chemical_compound Tetragonal crystal system Chlorides Structural Biology law Cations medicine Animals Crystallization Solubility chemistry.chemical_classification Temperature General Medicine chemistry Thermodynamics Muramidase Lysozyme medicine.drug |
| Zdroj: | Acta Crystallographica Section D Biological Crystallography. 58:1582-1587 |
| ISSN: | 0907-4449 |
| DOI: | 10.1107/s0907444902014518 |
| Popis: | The influence of salt nature and concentration on tetragonal lysozyme chloride crystal solubility is presented for a set of mono-, di- and trivalent cations (Cs+, Rb+, Mn2+, Co2+ and Yb3+). The results show that cations have as strong an effect on protein solubility as anions and that they present their own particular effects as co-ions. Indeed, after decreasing at low ionic strength, lysozyme solubility increases with high concentration of polyvalent cations, probably due to co-ion binding and therefore the concomitant increase of the net charge of the protein-salt complex. These new results are discussed in order to progress in the understanding of the crystallisation process at the atomic level. |
| Databáze: | OpenAIRE |
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