The secretion of aspartyl proteinase, a virulence enzyme, by isolates of Candida albicans from the oral cavity of HIV-infected subjects
| Autor: | Rainaldi L, Antonio Cassone, F De Bernardis, C. E. Guerra, Isabella Quinti, M. Boccanera |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
CD4-Positive T-Lymphocytes
Serotype Epidemiology Population Virulence HIV Infections Asymptomatic Microbiology Leukocyte Count Candidiasis Oral Candida albicans HIV Seropositivity Prevalence medicine Aspartic Acid Endopeptidases Humans education Mycosis Acquired Immunodeficiency Syndrome education.field_of_study biology business.industry Incidence Mouth Mucosa medicine.disease biology.organism_classification Virology Corpus albicans Italy Oral thrush medicine.symptom business |
| Zdroj: | European Journal of Epidemiology. 8:362-367 |
| ISSN: | 1573-7284 0393-2990 |
| Popis: | Prevalence, serotype and in vitro secretion of aspartyl proteinase, a virulence enzyme, were studied in Candida isolates from the oral cavity of 337 HIV-infected subjects. Controls were 95 age-sex-matched HIV- (seronegative) subjects, belonging to either HIV-risk categories (47) or to the normal, general population (48). Fungi were isolated from 155 HIV+ subjects. C. albicans was the most prevalent species (85.8% of all isolates). 94.6% of C. albicans isolates were serotype A and all were agglutinated by a monoclonal antibody (AF1) directed against a major mannoprotein immunogen of the candidal cell wall, confirming previous results with C. albicans isolates from non-immunodeficient subjects. With regard to the stage of HIV infection, there were no statistically significant differences in the incidence of oral Candida carriage between asymptomatic (stage II) HIV+ and HIV- subjects, and between stage II and lymphadenopathic (stage III) individuals. Also, the low (3.8%) incidence of oral candidiasis in the subjects of the latter stage was insignificant with respect to stage II subjects. However, the incidence of C. albicans in stage IV (AIDS) subjects (46.8%) was significantly higher than in all other subjects, and in almost all cases, fungal isolation was accompanied by oral thrush and lower CD4+ lymphocyte counts (less than 400 x 10(6)/L). All isolates of C. albicans were proteolytic in vitro, as assessed by scoring the proteinase activity on BSA agar and monitoring the secreted proteinase antigen by a highly sensitive (1 ng) and specific immunoenzymatic assay.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink