Clinical performance of the 3M Rapid Detection Flu A+B Test compared to R-Mix culture, DFA and BinaxNOW Influenza AB Test
| Autor: | Margaret Kowerska, Mark Bornfreund, Christine C. Ginocchio, Leon H. Falk, Suman Arora, Madhavi Lotlikar, Ryhana Manji |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent First line Rapid detection Gastroenterology Sensitivity and Specificity Young Adult Virology Internal medicine Influenza Human medicine Humans False Positive Reactions Child Direct fluorescent antibody Rapid response Aged Aged 80 and over business.industry Clinical performance Infant Newborn Infant Influenza a Middle Aged Orthomyxoviridae Patient management Infectious Diseases Molecular Diagnostic Techniques Child Preschool Immunology Healthcare settings Female Reagent Kits Diagnostic business |
| Zdroj: | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 45(2) |
| ISSN: | 1873-5967 |
| Popis: | The rapid diagnosis of influenza allows for prompt patient management and the initiation of appropriate infection control measures to reduce spread in healthcare settings.To evaluate the clinical performance of the 3M Rapid Detection Flu A+B Test (3MA+B) as compared to R-Mix cell culture, direct immunofluorescence assay (DFA) and the BinaxNOW AB Influenza Test (BinaxNOW).Five hundred fresh respiratory samples, collected from patients aged 5 days to 99 years with respiratory symptoms, were tested by R-Mix culture, DFA, 3MA+B and BinaxNOW. Analytical sensitivity of 3MA+B was compared to BinaxNOW using replicates of serially diluted clinical samples positive for influenza A or B.Sensitivity, specificity, PPV and NPV for the detection of influenza A and B, respectively, were for R-Mix (96.9%, 100%, 100%, 99.3%; 98.1%, 100%, 100%, 99.8%), DFA (80.4%, 99.2%, 96.1%, 95.3%; 74%, 100%, 100%, 97%), 3MA+B (70.1%, 99.8%, 98.6%, 93%; 86.5%, 98.7%, 88.2%, 98.4%) and BinaxNOW (46.4%, 100%, 100%, 88.6%; 34.6%, 100%, 100%, 93%). R-Mix, DFA and 3MA+B were significantly (Por=0.0001) more sensitive than BinaxNOW for the detection of both influenza A and B. The analytical sensitivity of 3MA+B was greater than BinaxNOW. Excessive blood in samples may cause 3MA+B false positive influenza B results.The 3MA+B provided superior results compared to BinaxNOW. The 3MA+B Reader eliminated user misinterpretation and provided quality control and result documentation. The improved sensitivity and easy of use makes 3MA+B an effective first line triage test for emergency departments, clinics and rapid response laboratories. |
| Databáze: | OpenAIRE |
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