Clinical experience with transfusion of leukocyte-poor platelet concentrates prepared by filtration with prostacyclin
| Autor: | JW Akkerman, HC van Prooijen, TI Riemens |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Blood transfusion Platelet Aggregation Lymphocyte medicine.medical_treatment Immunology Prostacyclin Cell Separation Platelet Transfusion Gastroenterology Biochemistry Platelet Adhesiveness Bleeding time Platelet adhesiveness Internal medicine medicine Leukocytes Humans Centrifugation Platelet Blood Transfusion medicine.diagnostic_test business.industry Platelet Count Cell Biology Hematology Epoprostenol Adenosine Diphosphate Leukemia Myeloid Acute medicine.anatomical_structure Platelet transfusion business Filtration medicine.drug |
| Zdroj: | Blood. 70:243-246 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v70.1.243.bloodjournal701243 |
| Popis: | Repeated transfusions with platelets from randomly selected donors lead to HLA alloimmunization in about 50% of patients due to lymphocyte contamination of platelet concentrates. Attempts to remove the leukocytes from the platelet concentrates by additional centrifugation steps led to substantial loss of platelets. We report a new procedure for removal of almost all leukocytes with excellent platelet recoveries. Single donor concentrates are treated with 50 ng/mL prostacyclin to inactivate the platelets transiently. The concentrates are then passed through a cellulose-acetate filter to remove the leukocytes. In 30 concentrates this treatment reduced the contamination by leukocytes to less than 0.1 million per concentrate with a platelet recovery of 89% +/- 1% (mean +/- SEM). Thirty filtered platelet concentrates transfused to ten thrombocytopenic patients within one hour after filtration were well tolerated and led to corrected count increments of (22.0 +/- 1.1) X 10(6)/mL blood after one hour and normal survival thereafter. In four of five patients these concentrates reduced the bleeding time. We conclude that transient inactivation of platelets by prostacyclin enables optimal removal of leukocytes and may help to reduce alloimmunization during frequent transfusions with platelet concentrates. |
| Databáze: | OpenAIRE |
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