Phospholipase Cγ2 is necessary for separation of blood and lymphatic vasculature in mice
| Autor: | Taeko Ichise, Nobuaki Yoshida, Osamu Ohtani, Hirotake Ichise |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Mutant
Syk Mice Transgenic Phospholipase Biology Mice Pregnancy medicine Animals Humans Molecular Biology Cells Cultured Bone Marrow Transplantation DNA Primers Lymphatic Vessels Mice Knockout Polymorphism Genetic Base Sequence Phospholipase C gamma Endothelial Cells Phenotype Null allele Mice Mutant Strains Cell biology Lymphatic system medicine.anatomical_structure Vascular endothelial growth factor C Radiation Chimera Immunology Blood Vessels Female Bone marrow Developmental Biology |
| Zdroj: | Development. 136:191-195 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.025353 |
| Popis: | The lymphatic vasculature originates from the blood vasculature through a mechanism relying on Prox1 expression and VEGFC signalling, and is separated and kept separate from the blood vasculature in a Syk- and SLP76-dependent manner. However, the mechanism by which lymphatic vessels are separated from blood vessels is not known. To gain an understanding of the vascular partitioning, we searched for the affected gene in a spontaneous mouse mutant exhibiting blood-filled lymphatic vessels, and identified a null mutation of the Plcg2 gene, which encodes phospholipase Cgamma2 (PLCgamma2), by positional candidate cloning. The blood-lymph shunt observed in PLCgamma2-null mice was due to aberrant separation of blood and lymphatic vessels. A similar phenotype was observed in lethally irradiated wild-type mice reconstituted with PLCgamma2-null bone marrow cells. These findings indicate that PLCgamma2 plays an essential role in initiating and maintaining the separation of the blood and lymphatic vasculature. |
| Databáze: | OpenAIRE |
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