Stem cell behavior on tailored porous oxide surface coatings
| Autor: | Richard W. Siegel, David J. Poxson, Sandrine Lavenus, Nika Ogievetsky, Jonathan S. Dordick |
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| Rok vydání: | 2015 |
| Předmět: |
Materials science
Surface Properties Silicon dioxide Cell Culture Techniques Biophysics Biocompatible Materials Bioengineering Nanotechnology Cell Separation engineering.material Microscopy Atomic Force Biomaterials chemistry.chemical_compound Coating Cell Adhesion Humans Nanotopography Porosity Nanoscopic scale Cell Proliferation Titanium Reverse Transcriptase Polymerase Chain Reaction Mesenchymal stem cell technology industry and agriculture Cell Differentiation Mesenchymal Stem Cells Flow Cytometry Silicon Dioxide Immunohistochemistry Nanostructures Microscopy Fluorescence chemistry Mechanics of Materials Physical vapor deposition Ceramics and Composites engineering Stem cell Signal Transduction |
| Zdroj: | Biomaterials. 55:96-109 |
| ISSN: | 0142-9612 |
| Popis: | Nanoscale surface topographies are known to have a profound influence on cell behavior, including cell guidance, migration, morphology, proliferation, and differentiation. In this study, we have observed the behavior of human mesenchymal stem cells cultured on a range of tailored porous SiO2 and TiO2 nanostructured surface coatings fabricated via glancing angle electron-beam deposition. By controlling the physical vapor deposition angle during fabrication, we could control systematically the deposited coating porosity, along with associated topographic features. Immunocytochemistry and image analysis quantitatively revealed the number of adherent cells, as well as their basic cellular morphology, on these surfaces. Signaling pathway studies showed that even with subtle changes in nanoscale surface structures, the behavior of mesenchymal stem cells was strongly influenced by the precise surface structures of these porous coatings. |
| Databáze: | OpenAIRE |
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