Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis
| Autor: | Michael G. Ehrlich, Hans J. Barrach, Timothy E. Radomisli, Hugh Keeping, Douglas C. Moore |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Osteocalcin Osteogenesis Distraction Bone Morphogenetic Protein 2 In situ hybridization Bone Morphogenetic Protein 4 medicine.disease_cause Bone morphogenetic protein 2 Collagen Type I Weight-bearing Rats Sprague-Dawley Weight-Bearing Transforming Growth Factor beta Internal medicine medicine Animals Orthopedics and Sports Medicine Collagen Type II biology Chemistry Anatomy Immunohistochemistry Rats Endocrinology Bone morphogenetic protein 4 Intramembranous ossification Bone Morphogenetic Proteins biology.protein Distraction osteogenesis |
| Zdroj: | Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 19(6) |
| ISSN: | 0736-0266 |
| Popis: | This study was performed to investigate the effect of loading on the biology of newly forming bone during limb lengthening. Unilateral 2.0 mm femoral lengthenings were performed in 20 male Sprague Dawley rats. Half (n = 10) of the animals were allowed to bear weight freely, while the other half were prevented from weight-bearing via an ipsilateral through-knee amputation. The animals in each group were sacrificed after one (n = 5) or four (n = 5) days of consolidation (post-operative days seven and 10, respectively). In situ hybridization for osteocalcin and collagen I, and antibody staining for collagen II and BMP 2/4 were used to evaluate the molecular influence of loading. There was more new bone in the distraction gap of the weight-bearing animals than there was in the non-weight-bearing animals. BMP 2/4 expression, and the messages for collagen I and osteocalcin, were more abundant in tissue from the weight-bearing animals; collagen II was higher in the non-weight-bearing animals. This suggests that early regenerate tissue is capable of responding to loading, and that weight-bearing appears to stimulate intramembranous ossification. These findings support the concept of early weight-bearing after limb lengthening. |
| Databáze: | OpenAIRE |
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