Discovery of a Nitric Oxide Responsive Quorum Sensing Circuit in Vibrio cholerae
| Autor: | Kate E. Higgins, Elizabeth M. Boon, Bernadette M. Henares |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hemeproteins Histidine Kinase medicine.disease_cause Nitric Oxide Biochemistry Article Microbiology 03 medical and health sciences Bacterial Proteins medicine Phosphorylation Vibrio cholerae Regulation of gene expression 030102 biochemistry & molecular biology biology Kinase Vibrio harveyi Chemistry Autophosphorylation Biofilm Quorum Sensing General Medicine Gene Expression Regulation Bacterial biology.organism_classification Cell biology Quorum sensing 030104 developmental biology Molecular Medicine Autoinducer |
| Zdroj: | ACS chemical biology. 13(8) |
| ISSN: | 1554-8937 |
| Popis: | Group behavior of the human pathogen Vibrio cholerae, including biofilm formation and virulence factor secretion, is mediated by a process known as quorum sensing. Quorum sensing is a way by which bacteria coordinate gene expression in response to population density through the production, secretion, and detection of small molecules called autoinducers. Four autoinducer-mediated receptor histidine kinases have been implicated in quorum sensing through the phosphotransfer protein LuxU: CqsS, LuxP/Q, CqsR, and VpsS (Vc1445). Of these receptor kinases, VpsS is predicted to be cytosolic, and its cognate autoinducer is currently unknown. In this study, we demonstrate that the nitric oxide-bound complex of a member of the recently discovered family of nitric oxide-responsive hemoproteins called NosP (VcNosP is encoded by Vc1444; this gene product is also known as VpsV) inhibits the autophosphorylation activity of VpsS and thus phosphate flow to LuxU. Therefore, we propose that VpsS contributes to the regulation of quorum sensing in a nitric-oxide-dependent manner through its interaction with NosP. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|