Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature
Autor: | Jeffrey J. L. Carson, Barry G. H. Janssen, Mohamadreza Najiminaini, Parsa Omidi, Yan Min Zhang |
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Rok vydání: | 2021 |
Předmět: |
Fluorescence microscopy
0301 basic medicine Microscopy Fluorescence-lifetime imaging microscopy Materials science Microscope QH201-278.5 Bright-field microscopy Context (language use) General Medicine law.invention Intravital microscopy 03 medical and health sciences Technical Report 030104 developmental biology 0302 clinical medicine Optical microscope law Fluorescence microscope Multispectral microscopy Brightfield microscopy 030217 neurology & neurosurgery Biomedical engineering |
Zdroj: | Applied Microscopy Applied Microscopy, Vol 51, Iss 1, Pp 1-12 (2021) |
ISSN: | 2287-4445 |
DOI: | 10.1186/s42649-021-00059-6 |
Popis: | Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation. Supplementary Information The online version contains supplementary material available at 10.1186/s42649-021-00059-6. |
Databáze: | OpenAIRE |
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