Functionalization of Ultrabithorax Materials with Vascular Endothelial Growth Factor Enhances Angiogenic Activity
| Autor: | Sarah E. Bondos, Kayla J. Bayless, Shang-Pu Tsai, Camille L. Duran, David W. Howell |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A animal structures Polymers and Plastics Angiogenesis Neovascularization Physiologic Bioengineering Nanotechnology Chick Embryo Biology Biomaterials 03 medical and health sciences chemistry.chemical_compound Tissue engineering Cell Movement VEGF Signaling Pathway Materials Chemistry Human Umbilical Vein Endothelial Cells Morphogenesis Animals Drosophila Proteins Humans Ultrabithorax Homeodomain Proteins Tissue Engineering Tissue Scaffolds Fusion protein Cell biology Endothelial stem cell Vascular endothelial growth factor Chorioallantoic membrane 030104 developmental biology chemistry embryonic structures Chickens Signal Transduction Transcription Factors |
| Zdroj: | Biomacromolecules. 17(11) |
| ISSN: | 1526-4602 |
| Popis: | Successful design of tissue engineering scaffolds must include the ability to stimulate vascular development by incorporating angiogenic growth factors. Current approaches can allow diffusion of growth factors, incorporate active factors randomly, or can leave residual toxins. We addressed these problems by genetically fusing the gene encoding Vascular Endothelial Growth Factor (VEGF) with the Ultrabithorax (Ubx) gene to produce fusion proteins capable of self-assembly into materials. We demonstrate that VEGF-Ubx materials enhance human endothelial cell migration, prolong cell survival, and dose-dependently activate the VEGF signaling pathway. VEGF-Ubx fibers attract outgrowing sprouts in an aortic ring assay and induce vessel formation in a chicken embryo chorioallantoic membrane (CAM) assay. Collectively, these results demonstrate that the activity of VEGF remains intact in Ubx materials. This approach could provide an inexpensive and facile mechanism to stimulate and pattern angiogenesis. |
| Databáze: | OpenAIRE |
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