A rapid method combining immunofluorescence and flow cytometry for improved understanding of competitive interactions between lactic acid bacteria (LAB) and methicillin-resistant S. aureus (MRSA) in mixed culture
| Autor: | Barbara Karska-Wysocki, Wanda Smoragiewicz, John Schellenberg |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Population Fluorescent Antibody Technique Biology medicine.disease_cause Microbiology Flow cytometry Agar plate chemistry.chemical_compound Antibiotic resistance Lactobacillus medicine Animals Humans education Fluorescein isothiocyanate Molecular Biology Bacteriological Techniques education.field_of_study medicine.diagnostic_test Probiotics Flow Cytometry biology.organism_classification Antibodies Bacterial Coculture Techniques Microscopy Fluorescence chemistry Methicillin Resistance Fluorescein-5-isothiocyanate Bacteria |
| Zdroj: | Journal of Microbiological Methods. 65:1-9 |
| ISSN: | 0167-7012 |
| Popis: | The increasing frequency of methicillin-resistant Staphylococcus aureus (MRSA) infections in hospital and community settings highlights the need for effective anti-MRSA agents that will not contribute to the growing problem of antibiotic resistance. Lactic acid bacteria (LAB) are known to exclude various pathogens through multiple mechanisms. In vitro models studying interactions of pathogens and LAB in mixed cultures use selective agar plates to quantify changes in target populations. We applied commercially available S. aureus-specific polyclonal antibodies conjugated with fluorescein isothiocyanate (FITC) for this purpose, producing a bright green signal that clearly differentiates S. aureus from LAB species when mixed cultures are analyzed by flow cytometry and fluorescent microscopy. Flow cytometry of mixed cultures revealed a much larger population of MRSA cells than was detectable using selective agar plates. To our knowledge, this is the first time immunofluorescent flow cytometry has been applied to the study of competitive exclusion in mixed bacterial populations over time. |
| Databáze: | OpenAIRE |
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