Generation of aMig-6 conditional null allele
| Autor: | Francesco J. DeMayo, Nili Jin, Russell Broaddus, Jae Wook Jeong, Jennifer L. Gilbert |
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| Rok vydání: | 2007 |
| Předmět: |
Mice
Knockout Intracellular Signaling Peptides and Proteins Gene targeting Cre recombinase Cell Biology Biology Molecular biology Null allele Mice Exon Endocrinology Epidermal growth factor Gene expression Conditional gene knockout Genetics Animals Allele Genetic Engineering Cell Shape Alleles Adaptor Proteins Signal Transducing Skin |
| Zdroj: | genesis. 45:716-721 |
| ISSN: | 1526-968X 1526-954X |
| DOI: | 10.1002/dvg.20348 |
| Popis: | Mitogen-inducible gene 6 (Mig-6) is a stress-induced gene that serves as a negative regulator of epidermal growth factor (EGF) signaling and acts as a tumor suppressor. Ablation of Mig-6 results in a significant percentage of embryo lethality as well as abnormalities in multiple tissues. To understand the physiological roles of Mig-6, a conditional null allele, Mig-6(f/f) was generated by introducing LoxP sites that flank exons 2 and 4. The Mig-6(f/f) allele was validated by generating recombined Mig-6(-/-) mice using the Zp3-Cre system. The conditional null allele was confirmed by assaying for Mig-6 gene expression in liver, lung, uterus, and skin. The recombined Mig-6(-/-) mice developed pathological changes, such as degenerative joint diseases and skin hyperplasia similar to the previously reported Mig-6 germline null allele. In addition, these mice also had enlarged uteri with endometrial hyperplasia. In summary, this Mig-6(f/f) mouse is a useful tool for the functional study of the Mig-6 gene in a tissue-specific fashion. |
| Databáze: | OpenAIRE |
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