Induction of functional interferon alpha and gamma responses during acute infection of cattle with non-cytopathic bovine viral diarrhea virus
| Autor: | Sylvia van Drunen Littel-van den Hurk, Brenden Van Wyk, Marlene Snider, Scott Napper, Erin Scruten |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Gene Expression Regulation
Viral 0301 basic medicine 040301 veterinary sciences Host–pathogen interaction Alpha interferon Biology Microbiology Peripheral blood mononuclear cell Virus 0403 veterinary science Interferon-gamma 03 medical and health sciences Blood serum Pregnancy Interferon medicine Animals Interferon gamma Diarrhea Viruses Bovine Viral General Veterinary Interferon-alpha 04 agricultural and veterinary sciences General Medicine Viral Load Virology 030104 developmental biology Immunology Bovine Virus Diarrhea-Mucosal Disease Cattle Female Viral load Signal Transduction medicine.drug |
| Zdroj: | Veterinary Microbiology. 195:104-114 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2016.09.015 |
| Popis: | As a part of their pathogenic mechanism, many pathogens causing persistent infections, including bovine viral diarrhea virus (BVDV), immunosuppress their hosts, often by limiting the ability to either produce, or respond to, interferon. The objective of this study was to quantify the extent to which an acute infection of cattle with a non-cytopathic strain of BVDV induces interferon responses and to establish the functionality of these responses. Functionality of responses was investigated using a bovine specific peptide array to monitor kinase-mediated signal transduction activity within peripheral blood mononuclear cells (PBMCs) at time points corresponding to the interferon gamma (IFN-γ) and alpha (IFN-α) responsive phases of acute BVDV infection. Further, with an appreciation of diverse mechanisms and levels at which pathogens modulate host cell defences, patterns of expression of IFN-γ and -α responsive genes were also quantified within PBMCs. Infection of cows with ncpBVDV2-1373 induced significant increases in levels of serum IFN-γ and IFN-α. Within the PBMCs of the infected animals, distinct patterns of kinase-mediated signal transduction activity, in particular with respect to activation of classic IFN-activated signalling pathways, such as Jak-Stat, as well as induced expression of IFN-γ and IFN-α regulated genes, support the functionality of the host interferon response. |
| Databáze: | OpenAIRE |
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