Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice
| Autor: | Rui Li, Shiyi Zhao, Xinxin Zhang, Hui-Ming Li, Qianzi Yang, Dan Wang, Hailong Dong, Huihui Li, Haixing Zhong, Juan Guo, Sa Wang, Tingting Tong, Ao Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Physiology Lateral hypothalamic area Optogenetics Electroencephalography Biology Photostimulation 03 medical and health sciences Glutamatergic Mice 0302 clinical medicine Lateral habenula medicine Animals Anesthesia GABAergic Neurons Anesthetics Habenula medicine.diagnostic_test Isoflurane General Neuroscience General Medicine Glutamatergic neuron Burst suppression 030104 developmental biology nervous system Hypothalamic Area Lateral Anesthetic GABAergic Original Article Neuroscience 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neuroscience Bulletin |
| ISSN: | 1995-8218 1673-7067 |
| Popis: | The lateral hypothalamic area (LHA) plays a pivotal role in regulating consciousness transition, in which orexinergic neurons, GABAergic neurons, and melanin-concentrating hormone neurons are involved. Glutamatergic neurons have a large population in the LHA, but their anesthesia-related effect has not been explored. Here, we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice. In contrast, chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery. Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram (EEG) and shifted EEG features to an arousal pattern. Photostimulation of LHA glutamatergic projections to the lateral habenula (LHb) also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level. Collectively, LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink