Pf4 bacteriophage produced by Pseudomonas aeruginosa inhibits Aspergillus fumigatus metabolism via iron sequestration
| Autor: | Karl V. Clemons, Jose A. G. Ferreira, Maria Birukova, David A. Stevens, Janus A. J. Haagensen, John C. Penner, Hasan Nazik, Johanna M. Sweere, Patrick R. Secor, Alfred M. Spormann, Andrey V. Malkovskiy, Paul L. Bollyky, Robert Manasherob, Omar Garcia, Gernot Kaber, Lydia-Marie Joubert |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Hypha Pseudomonas aeruginosa Aspergillus fumigatus Iron 030106 microbiology Biofilm Fungus Metabolism biology.organism_classification medicine.disease_cause Microbiology Bacteriophage 03 medical and health sciences Biofilms medicine Aspergillosis Humans Bacteriophages Bacteria |
| Zdroj: | Microbiology (Reading, England). 162(9) |
| ISSN: | 1465-2080 |
| Popis: | Pseudomonas aeruginosa (Pa) and Aspergillus fumigatus (Af) are major human pathogens known to interact in a variety of disease settings, including airway infections in cystic fibrosis. We recently reported that clinical CF isolates of Pa inhibit the formation and growth of Af biofilms. Here, we report that the bacteriophage Pf4, produced by Pa, can inhibit the metabolic activity of Af biofilms. This phage-mediated inhibition was dose dependent, ablated by phage denaturation, and was more pronounced against preformed Af biofilm rather than biofilm formation. In contrast, planktonic conidial growth was unaffected. Two other phages, Pf1 and fd, did not inhibit Af, nor did supernatant from a Pa strain incapable of producing Pf4. Pf4, but not Pf1, attaches to Af hyphae in an avid and prolonged manner, suggesting that Pf4-mediated inhibition of Af may occur at the biofilm surface. We show that Pf4 binds iron, thus denying Af a crucial resource. Consistent with this, the inhibition of Af metabolism by Pf4 could be overcome with supplemental ferric iron, with preformed biofilm more resistant to reversal. To our knowledge, this is the first report of a bacterium producing a phage that inhibits the growth of a fungus and the first description of a phage behaving as an iron chelator in a biological system. |
| Databáze: | OpenAIRE |
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