High-throughput quantitative microscopy-based half-life measurements of intravenously injected agents
| Autor: | Alexandra S. Piotrowski-Daspit, Chun-Yu Lin, Gregory T. Tietjen, Yongheng Wang, W. Mark Saltzman, Laura G. Bracaglia, Zoe M. Moscato |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Mice Inbred BALB C Microscopy Multidisciplinary Chemistry Half-life Blood volume Biological Sciences Polymeric nanoparticles High-Throughput Screening Assays Mice Blood concentration Pharmaceutical Preparations Quantitative Microscopy Drug delivery Injections Intravenous Animals Humans Nanoparticles Female Drug Monitoring Throughput (business) Biomedical engineering Half-Life |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 |
| Popis: | Accurate analysis of blood concentration and circulation half-life is an important consideration for any intravenously administered agent in preclinical development or for therapeutic application. However, the currently available tools to measure these parameters are laborious, expensive, and inefficient for handling multiple samples from complex multivariable experiments. Here we describe a robust high-throughput quantitative microscopy-based method to measure the blood concentration and circulation half-life of any fluorescently labeled agent using only a small (2 µL) amount of blood volume, enabling additional end-point measurements to be assessed in the same subject. To validate this method, we demonstrate its use to measure the circulation half-life in mice of two types of fluorescently labeled polymeric nanoparticles of different sizes and surface chemistries and of a much smaller fluorescently labeled monoclonal antibody. Furthermore, we demonstrate the improved accuracy of this method compared to previously described methods. |
| Databáze: | OpenAIRE |
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