Genotyping and antifungal susceptibility of human subgingival Candida albicans isolates
| Autor: | Francesco Barchiesi, D. Arzeni, L Falconi Di Francesco, Maria Eleonora Milici, G. Scalise, Matteo D'Angelo, Giovanna Giuliana, Giuseppi Pizzo |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Posaconazole Antifungal Agents Itraconazole Dental Plaque HIV Infections Microbial Sensitivity Tests Microbiology Periodontal pathogen Genetic Heterogeneity Drug Resistance Fungal Amphotericin B Genotype Candida albicans medicine Humans DNA Fungal Mycological Typing Techniques General Dentistry Genotyping Fluconazole biology Cell Biology General Medicine Triazoles biology.organism_classification Virology Corpus albicans Otorhinolaryngology Karyotyping Female medicine.drug |
| Zdroj: | Archives of oral biology. 47(3) |
| ISSN: | 0003-9969 |
| Popis: | Subgingival colonization by Candida albicans has been described in human immunodeficiency virus (HIV)-infected individuals, but subgingival isolates have scarcely been characterized, particularly with respect to genotype and antifungal susceptibility. A series of 29 subgingival strains of C. albicans isolated from nine HIV-infected individuals was typed by electrophoretic karyotyping and tested for susceptibility to fluconazole, itraconazole, the new investigational triazole posaconazole and amphotericin B. DNA typing showed genetic heterogeneity within subgingival isolates, as almost every individual harbored his/her own specific isolate. Genetic identity was usually demonstrated within oral and subgingival isolates simultaneously collected from the same individual, but a number of DNA types were found to be unique to subgingival strains. These findings suggest that colonization is not just the result of Candida spreading from oral surfaces, and that subgingivally adapted strains could be involved. All isolates were susceptible to all the triazole drugs tested and amphotericin B. Additional studies on subgingival Candida colonization and further characterization of subgingival isolates are now required to clarify the role of Candida as opportunistic periodontal pathogen. |
| Databáze: | OpenAIRE |
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