Expression of Genes Related to Phenylpropanoid Biosynthesis in Different Organs of Ixeris dentata var. albiflora
| Autor: | Sanghoon Lee, Chan-Sik Jung, Seong-Cheol Kim, Yeon Bok Kim, Yoonkang Hur, Sang Won Lee, Jae-Ki Jang, Yun-Ji Park, Sang Un Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chalcone synthase Propanols Pharmaceutical Science Ixeris dentata var. albiflora phenylpropanoid transcript level Biology Asteraceae Real-Time Polymerase Chain Reaction 01 natural sciences Article Analytical Chemistry Ferulic acid lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound Chlorogenic acid Biosynthesis lcsh:Organic chemistry Gene Expression Regulation Plant Drug Discovery Botany Physical and Theoretical Chemistry Phenylalanine Ammonia-Lyase Plant Proteins Ixeris Phenylpropanoid Organic Chemistry food and beverages Catechin biology.organism_classification 030104 developmental biology chemistry Biochemistry Chemistry (miscellaneous) biology.protein Molecular Medicine Quercetin Acyltransferases 010606 plant biology & botany |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules, Vol 22, Iss 6, p 901 (2017) Molecules; Volume 22; Issue 6; Pages: 901 |
| ISSN: | 1420-3049 |
| Popis: | Members of the genus Ixeris have long been used in traditional medicines as stomachics, sedatives, and diuretics. Phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate: coenzyme-A (CoA) ligase (4CL), chalcone synthase (CHS), and dihydroflavonol 4-reductase (DFR) are important enzymes in the phenylpropanoid pathway. In this study, we analyzed seven genes from Ixeris dentata var. albiflora that are involved in phenylpropanoid biosynthesis, using an Illumina/Solexa HiSeq 2000 platform. The amino acid sequence alignments for IdPALs, IdC4H, Id4CLs, IdCHS, and IdDFR showed high identity to sequences from other plants. We also investigated transcript levels using quantitative real-time PCR, and analyzed the accumulation of phenylpropanoids in different organs of I. dentata var. albiflora using high-performance liquid chromatography. The transcript levels of IdC4H, Id4CL1, IdCHS, and IdDFR were highest in the leaf. The catechin, chlorogenic acid, ferulic acid, and quercetin contents were also highest in the leaf. We suggest that expression of IdC4H, Id4CL1, IdCHS, and IdDFR is associated with the accumulation of phenylpropanoids. Our results may provide baseline information for elucidating the mechanism of phenylpropanoid biosynthesis in different organs of I. dentata var. albiflora. |
| Databáze: | OpenAIRE |
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