Direct-tissue SELDI-TOF mass spectrometry analysis: a new application for clinical proteomics
| Autor: | Jean-Paul Issartel, François Berger, David Ratel, Elisabeth Brambilla, Jessica Ternier, Alim-Louis Benabid, Ali Bouamrani |
|---|---|
| Přispěvatelé: | Neurosciences précliniques, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de neurochirurgie, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Département d'anatomie et cythologie pathologique, CHU Grenoble-Hôpital Michallon, Issartel, Jean-Paul |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Proteomics
Pathology medicine.medical_specialty Oligodendroglioma Clinical Biochemistry Mouse tissue Mass spectrometry Protein expression MESH: Central Nervous System Neoplasms Central Nervous System Neoplasms Mice 03 medical and health sciences 0302 clinical medicine Seldi tof Biopsy medicine Animals Humans MESH: Animals MESH: Mice MESH: Oligodendroglioma MESH: Organ Specificity 030304 developmental biology 0303 health sciences MESH: Humans medicine.diagnostic_test Proteomic Profiling Chemistry MESH: Glioblastoma MESH: Proteomics Biochemistry (medical) MESH: Biological Markers MESH: Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Organ Specificity Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization 030220 oncology & carcinogenesis Glioblastoma Biomarkers Human cancer |
| Zdroj: | Clinical Chemistry Clinical Chemistry, American Association for Clinical Chemistry, 2006, 52 (11), pp.2103-6. ⟨10.1373/clinchem.2006.070979⟩ |
| ISSN: | 0009-9147 1530-8561 |
| DOI: | 10.1373/clinchem.2006.070979⟩ |
| Popis: | Background: New molecular profiling technologies can aid in analysis of small pathologic samples obtained by minimally invasive biopsy and may enable the discovery of key biomarkers synergistic with anatomopathologic analysis related to prognosis, therapeutic response, and innovative target validation. Thus proteomic analysis at the histologic level in healthy and pathologic settings is a major issue in the field of clinical proteomics. Methods: We used surface-enhanced laser desorption ionization-time-of-flight mass spectrometry (SELDI-TOF MS) technology with surface chromatographic subproteome enrichment and preservation of the spatial distribution of proteomic patterns to detect discrete modifications of protein expression. We performed in situ proteomic profiling of mouse tissue and samples of human cancer tissue, including brain and lung cancer. Results: This approach permitted the discrimination of glioblastomas from oligodendrogliomas and led to the identification of 3 potential markers. Conclusion: Direct tissue proteomic analysis is an original application of SELDI-TOF MS technology that can expand the use of clinical proteomics as a complement to the anatomopathological diagnosis. |
| Databáze: | OpenAIRE |
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