The presence of high-risk human papillomavirus (HPV) E6/E7 mRNA transcripts in a subset of sinonasal carcinomas is evidence of involvement of HPV in its etiopathogenesis
| Autor: | Luboš Tuček, Petr Čelakovský, Milan Vošmik, Ales Ryska, Radovan Slezák, Kristýna Němejcová, Viktor Chrobok, Jan Laco, Chmelařová M, Hana Vošmiková, Igor Sirák, Kateřina Sieglová, Alena Mottlová, Jaroslav Michalek, Radovan Mottl, Pavel Dundr |
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| Rok vydání: | 2015 |
| Předmět: |
Nasal cavity
Adult Male Pathology medicine.medical_specialty Maxillary sinus Transcription Genetic Papillomavirus E7 Proteins In situ hybridization Biology Pathology and Forensic Medicine law.invention law medicine Humans RNA Messenger Human papillomavirus Molecular Biology Papillomaviridae Polymerase chain reaction Cyclin-Dependent Kinase Inhibitor p16 Aged Aged 80 and over Messenger RNA Surrogate endpoint Reverse Transcriptase Polymerase Chain Reaction Squamous Cell Carcinoma of Head and Neck Cell Biology General Medicine Oncogene Proteins Viral Middle Aged Immunohistochemistry Neoplasm Proteins Repressor Proteins stomatognathic diseases medicine.anatomical_structure Head and Neck Neoplasms DNA Viral Carcinoma Squamous Cell Female Paranasal Sinus Neoplasms |
| Zdroj: | Virchows Archiv : an international journal of pathology. 467(4) |
| ISSN: | 1432-2307 |
| Popis: | The aim of the study was to investigate prevalence of high-risk human papillomavirus (HR-HPV) infection in sinonasal carcinomas by immunohistochemistry, in situ hybridization, and polymerase chain reaction, detecting p16INK4a protein (p16) expression and presence of both HPV DNA and HPV E6/E7 messenger RNA (mRNA). The study comprised 47 males and 26 females, aged 23–83 years (median 62 years), mostly (67 %) with a squamous cell carcinoma (SCC). Of the tumors, 53 % arose in the nasal cavity, 42 % in the maxillary sinus, and 5 % in the ethmoid complex. The follow-up period ranged 1–241 months (median 19 months). HPV16, HPV18, or HPV35 were detected in 18/73 (25 %) tumors, 17 SCCs, and 1 small cell neuroendocrine carcinoma. There was a strong correlation between results of HPV detection methods and p16 expression (p |
| Databáze: | OpenAIRE |
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