Establishment of a Set of Combined Immunodeficient DA/Slc-Foxn1rnu Lystbg Congenic Rat Strains
| Autor: | Masayuki Mori, Toshihiko Gonda, Hiromitsu Kimura, Hidekazu Asai, Katsunori Sato, Norio Masui, Tetsu Nishikawa, Yumie Takagi, Makoto Yanabe, Shigeo Yokose |
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| Rok vydání: | 2004 |
| Předmět: |
T-Lymphocytes
Xenotransplantation medicine.medical_treatment Congenic Biology Polymerase Chain Reaction Rats Mutant Strains General Biochemistry Genetics and Molecular Biology law.invention Immune system Animals Congenic law medicine Animals Gene Polymerase chain reaction Genetics Severe combined immunodeficiency integumentary system General Veterinary Proteins Forkhead Transcription Factors General Medicine medicine.disease Molecular biology Phenotype Rats DNA-Binding Proteins Killer Cells Natural Transplantation Disease Models Animal Severe Combined Immunodeficiency Animal Science and Zoology Chediak-Higashi Syndrome Polymorphism Restriction Fragment Length Transcription Factors |
| Zdroj: | Experimental Animals. 53:399-407 |
| ISSN: | 1881-7122 1341-1357 |
| DOI: | 10.1538/expanim.53.399 |
| Popis: | The congenitally athymic nude rat is used for studying cancer and transplantation owing to its hairlessness and T-cell defective function caused by the Foxn1(rnu) gene. However, NK cell activity of the nude rat is markedly increased. It is known that NK cells play a major role in rejection of xenografts and in cytotoxicity against tumor cells. Thus, the athymic nude rat with impaired NK cell activity should be a useful model for extensive studies. The DA-Lyst(bg)/Lyst(bg) rat, a model for human Chediak-Higashi syndrome (CHS) is characterized by diluted-coat color and impairment of NK cell activity. We planned to establish a combined immunodeficient double mutant rat introgressed with the Foxn1(rnu) and Lyst(bg) genes and a set of congenic strains having an identical genetic backgrounds simultaneously. Based on the phenotypic and genetic characteristics of the parental rat strains, the new strains were produced using continuous backcross and diagnosis with molecular genetic techniques. Each disease gene was diagnosed with PCR-RFLP or the long-nested PCR method. Furthermore, we used a marker-assisted congenic strategy based on scanning the genetic backgrounds of the parental rats with 461 rat microsatellite markers. We think that the newly established DA/Slc-Foxn1(rnu)/Foxn1(rnu) Lyst(bg)/Lyst(bg) double mutant will be useful as a severe disease model for human CHS, and the set of DA/Slc-Foxn1(rnu) Lyst(bg) congenic strains which have impaired NK cell activity and/or defective T cell function should be useful for studying in cancer research, xenotransplantation, immune function and other wide-ranging studies. |
| Databáze: | OpenAIRE |
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