Foot-and-mouth disease virus 5'-terminal S fragment is required for replication and modulation of the innate immune response in host cells
| Autor: | Anna Kloc, Elizabeth Rieder, Mary Kenney, Elizabeth A. Schafer, Devendra K. Rai, Fayna Diaz-San Segundo, Teresa de los Santos |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Untranslated region Picornavirus animal diseases viruses Mutant Virus Replication Virus Cell Line 03 medical and health sciences Immune system Immunity Virology Cricetinae Animals Sequence Deletion Innate immune system biology virus diseases biochemical phenomena metabolism and nutrition biology.organism_classification Immunity Innate 030104 developmental biology Foot-and-Mouth Disease Virus RNA Viral Cattle Foot-and-mouth disease virus 5' Untranslated Regions |
| Zdroj: | Virology. 512 |
| ISSN: | 1096-0341 |
| Popis: | The S fragment of the FMDV 5’ UTR is predicted to fold into a long stem-loop structure and it has been implicated in virus-host protein interactions. In this study, we report the minimal S fragment sequence required for virus viability and show a direct correlation between the extent of the S fragment deletion mutations and attenuated phenotypes. Furthermore, we provide novel insight into the role of the S fragment in modulating the host innate immune response. Importantly, in an FMDV mouse model system, all animals survive the inoculation with the live A 24 FMDV-S 4 mutant, containing a 164 nucleotide deletion in the upper S fragment loop, at a dose 1000 higher than the one causing lethality by parental A 24 FMDV, indicating that the A 24 FMDV-S 4 virus is highly attenuated in vivo . Additionally, mice exposed to high doses of live A 24 FMDV-S 4 virus are fully protected when challenged with parental A 24 FMDV virus. |
| Databáze: | OpenAIRE |
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