General expression profiles of human native odontoblasts and pulp-derived cultured odontoblast-like cells are similar but reveal differential neuropeptide expression levels
| Autor: | Tuula Salo, Leo Tjäderhane, Florence Carrouel, Virve Pääkkönen, Françoise Bleicher, Jussi Vuoristo, Heiko Peters, Henry Magloire, Marie-Lise Couble, Ilka Wappler |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Adolescent Galanin Protein tyrosine phosphatase Biology Transcriptome Young Adult stomatognathic system Gene expression Humans Somatostatin receptor 1 Receptors Somatostatin Protein Precursors General Dentistry Cells Cultured Dental Pulp Odontoblasts Receptor-Like Protein Tyrosine Phosphatases Class 5 Microarray analysis techniques Gene Expression Profiling Neuropeptides Cell Biology General Medicine Microarray Analysis Molecular biology Gene expression profiling Odontoblast Otorhinolaryngology Receptors Opioid Pulp (tooth) Female Signal Transduction |
| Zdroj: | Archives of Oral Biology. 54:55-62 |
| ISSN: | 0003-9969 |
| Popis: | Odontoblasts play a central role during the dentin formation by organic matrix production and mineralisation. Recently, suitable in vitro techniques for studying mature primary odontoblasts and the newly differentiated odontoblasts have been developed. Firstly, the gene expression profiles of native and cultured odontoblasts were compared at large-scale to investigate the similarities and differences between the samples. Secondly, differential expression levels of the genes encoding neuronal proteins were analyzed to study odontoblasts sensory function.Microarray analysis was performed to mature native and cultured pulp-derived odontoblast-like cells to compare their transcriptome. Then, the probes positive only in one sample were divided into gene ontology categories. Expression levels of selected neuronal proteins were further studied with quantitative PCR, and at the protein level by immunofluorescence of mature and newly differentiated odontoblasts in developing tooth.Remarkable similarities between the general and neuronal protein gene expression profiles were observed. Higher cortistatin, galanin, somatostatin receptor 1 (SSTR1) and tyrosine phosphatase receptor type Z1 (PTPRZ1) expression was detected in native than in cultured odontoblast at the mRNA level. Pronociceptin was more abundantly expressed in cultured than in native odontoblasts. Immunofluorescence of mature and newly differentiated odontoblasts on human tooth germs confirmed the results.Cultured odontoblasts used in this study have similar general gene expression pattern to native odontoblasts, and therefore offer a valuable tool for the in vitro odontoblast studies. The expression of PTPRZ1 and galanin, which participate in sensory signal transduction, supports the previously suggested role of odontoblasts as sensory cells. |
| Databáze: | OpenAIRE |
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