CD40 Ligation on Human Cord Blood CD34+Hematopoietic Progenitors Induces Their Proliferation and Differentiation into Functional Dendritic Cells
| Autor: | Pia Björck, Sem Saeland, Leopoldo Flores-Romo, V Duvert, Cees van Kooten, Jacques Banchereau |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
T cell
Immunology Antigen presentation Antigens CD34 Biology Article Cell Line Mice medicine Animals Humans Immunology and Allergy Cytotoxic T cell Cell Lineage CD40 Antigens Antigen-presenting cell B cell Follicular dendritic cells Cell Differentiation Articles Dendritic Cells Dendritic cell Fetal Blood Hematopoietic Stem Cells Natural killer T cell Cell biology medicine.anatomical_structure Cell Division |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Human CD34+ multilineage progenitor cells (CD34HPC) from cord blood and bone marrow express CD40, a member of the tumor necrosis factor–receptor family present on various hematopoietic and nonhematopoietic cells. As hyper-IgM patients with mutated CD40 ligand (CD40L) exhibit neutropenia, no B cell memory, and altered T cell functions leading to severe infections, we investigated the potential role of CD40 on CD34HPC development. CD40activated cord blood CD34HPC were found to proliferate and differentiate independently of granulocyte/macrophage colony-stimulating factor, into a cell population with prominent dendritic cell (DC) attributes including priming of allogeneic naive T cells. DC generated via the CD40 pathway displayed strong major histocompatibility complex class II DR but lacked detectable CD1a and CD40 expression. These features were shared by a dendritic population identified in situ in tonsillar T cell areas. Taken together, the present data demonstrate that CD40 is functional on CD34HPC and its cross-linking by CD40L+ cells results in the generation of DC that may prime immune reactions during antigen-driven responses to pathogenic invasion, thus providing a link between hematopoiesis, innate, and adaptive immunity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|