Post-translational modifications in tumor biomarkers: the next challenge for aptamers?
| Autor: | María Jesús Lobo-Castañón, Ana Díaz-Fernández, Noemí de-los-Santos-Álvarez, Rebeca Miranda-Castro |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Models Molecular Glycan Glycosylation Aptamer Computational biology Biosensing Techniques Proteomics Biochemistry Analytical Chemistry Abnormal glycosylation 03 medical and health sciences chemistry.chemical_compound Polysaccharides Neoplasms Biomarkers Tumor Animals Humans chemistry.chemical_classification biology SELEX Aptamer Technique Aptamers Nucleotide 030104 developmental biology chemistry biology.protein Biomarker (medicine) Cancer biomarkers Glycoprotein Protein Processing Post-Translational |
| Zdroj: | Scopus RUO. Repositorio Institucional de la Universidad de Oviedo instname |
| ISSN: | 1618-2650 |
| Popis: | Advances in proteomics have fueled the search for novel cancer biomarkers with higher selectivity. Differential expression of low abundant proteins has been the usual way of finding those biomarkers. The existence of a selective receptor for each biomarker is compulsory for their use in diagnostic/prognostic assays. Antibodies are the receptors of choice in most cases although aptamers are becoming familiar because of their facile and reproducible synthesis, chemical stability as well as comparable affinity and selectivity. In recent years, it has been reported that the pattern of post-translational modifications, altered under neoplastic disease, is a better predictive biomarker than the total protein level. Among others, abnormal glycosylation is attracting great attention. Lectins and antibodies are being used for identification and detection of the carbohydrate moiety with low level of discrimination among various glycoproteins. Such level of selectivity is critical to bring next-generation biomarkers to the clinic. Aptamers that can be rationally tailored for a certain molecule domain can become the golden receptor to specifically detect aberrant glycosylation at each protein or even at each glycosylation site, providing new diagnostic tools for early detection of cancer. Graphical abstract Aptamers may specifically differentiate normal from aberrant glycoproteins. |
| Databáze: | OpenAIRE |
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