Preliminary observations on expression of transforming growth factors [beta ]1 and [beta ]3 in equine full-thickness skin wounds healing normally or with exuberant granulation tissue
| Autor: | Spencer M. Barber, Christine L. Theoret, John R. Gordon, Terence N. Moyana |
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| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Transforming Growth Factor beta1 Transforming Growth Factor beta2 Transforming Growth Factor beta medicine Full thickness skin Animals Clinical significance Exuberant granulation tissue Horses Skin Wound Healing integumentary system General Veterinary business.industry Growth factor Horse Extremities Molecular control Bandages medicine.anatomical_structure Granulation Tissue Wounds and Injuries Forelimb business Transforming growth factor |
| Zdroj: | Veterinary Surgery. 31:266-273 |
| ISSN: | 0161-3499 |
| DOI: | 10.1053/jvet.2002.32394 |
| Popis: | OBJECTIVE To determine whether transforming growth factor (TGF)-beta1 and -beta3 expression differs between equine limb wounds healing normally and those healing with experimentally induced exuberant granulation tissue (EGT). STUDY DESIGN Six wounds were created on the lateral aspect of both metacarpi of each horse; one forelimb was untreated, and the other was bandaged to stimulate the development of EGT. Sequential wound biopsies allowed comparison of growth factor expression between the two types of wound. ANIMALS Four horses (2 to 4 years of age; 350 to 420 kg). METHODS Wounds were assessed grossly, histologically, and by enzyme-linked immunosorbent assay (ELISA) for TGF-beta1 and -beta3 expression at 12 and 24 hours and 2, 5, 10, and 14 days postoperatively. RESULTS Bandaged wounds developed EGT. In all wounds, TGF-beta1 peaked early and remained elevated at 14 days. Peak TGF-beta1 concentration was higher in wounds with EGT, but not significantly so. Expression of TGF-beta3 differed from TGF-beta1, with peak TGF-beta3 concentrations being delayed. Concentrations of TGF-beta3 were higher in wounds healing normally, but this difference was not significant. CONCLUSIONS During both normal and exuberant wound repair, the expression of TGF-beta1 occurred earlier than TGF-beta3 expression. Wounds healing with EGT tended to have higher concentrations of fibrogenic TGF-beta1 and lower concentrations of antifibrotic TGF-beta3 than wounds healing normally, although these differences were not statistically significant. CLINICAL RELEVANCE This study suggests that the production of EGT in bandaged wounds may be related to increased expression of fibrogenic TGF-beta1 and decreased expression of antifibrotic TGF-beta3. Further investigation of the roles of TGF-beta1 and -beta3 may be important in understanding the molecular control of EGT in horses. |
| Databáze: | OpenAIRE |
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