High-Resolution Transcript Profiling of the Atypical Biotrophic Interaction betweenTheobroma cacaoand the Fungal PathogenMoniliophthora perniciosa
| Autor: | Piotr A. Mieczkowski, Daniela P. T. Thomazella, Osvaldo Reis, Gabriel L. Fiorin, Maria Carolina Scatolin do Rio, Gustavo G.L. Costa, Gonçalo A.G. Pereira, Jorge Maurício Costa Mondego, Paulo José Pereira Lima Teixeira, Paula Favoretti Vital do Prado, Juliana José, Victor Augusti Negri |
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| Rok vydání: | 2014 |
| Předmět: |
Theobroma
Molecular Sequence Data Moniliophthora Virulence Plant Science Fungus Models Biological In Brief Moniliophthora perniciosa Microbiology Transcriptome Gene Expression Regulation Plant Gene Expression Regulation Fungal Cluster Analysis Large-Scale Biology Article Photosynthesis Pathogen Plant Diseases Plant Proteins Cacao Base Sequence Mycelium biology Sequence Analysis RNA Effector Gene Expression Profiling High-Throughput Nucleotide Sequencing Cell Biology biology.organism_classification Host-Pathogen Interactions Agaricales |
| Zdroj: | The Plant Cell. 26:4245-4269 |
| ISSN: | 1532-298X 1040-4651 |
| Popis: | Witches’ broom disease (WBD), caused by the hemibiotrophic fungus Moniliophthora perniciosa, is one of the most devastating diseases of Theobroma cacao, the chocolate tree. In contrast to other hemibiotrophic interactions, the WBD biotrophic stage lasts for months and is responsible for the most distinctive symptoms of the disease, which comprise drastic morphological changes in the infected shoots. Here, we used the dual RNA-seq approach to simultaneously assess the transcriptomes of cacao and M. perniciosa during their peculiar biotrophic interaction. Infection with M. perniciosa triggers massive metabolic reprogramming in the diseased tissues. Although apparently vigorous, the infected shoots are energetically expensive structures characterized by the induction of ineffective defense responses and by a clear carbon deprivation signature. Remarkably, the infection culminates in the establishment of a senescence process in the host, which signals the end of the WBD biotrophic stage. We analyzed the pathogen's transcriptome in unprecedented detail and thereby characterized the fungal nutritional and infection strategies during WBD and identified putative virulence effectors. Interestingly, M. perniciosa biotrophic mycelia develop as long-term parasites that orchestrate changes in plant metabolism to increase the availability of soluble nutrients before plant death. Collectively, our results provide unique insight into an intriguing tropical disease and advance our understanding of the development of (hemi)biotrophic plant-pathogen interactions. |
| Databáze: | OpenAIRE |
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