Nuclear Factor Binding Sites in Human βGlobin IVS2
| Autor: | D O'Neill, Arthur Bank, C E Jackson |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping DNA Footprinting Biology Biochemistry DNA-binding protein Antibodies Cell Line Mice Consensus Sequence Animals Humans Electrophoretic mobility shift assay Binding site Beta (finance) Molecular Biology Binding Sites Base Sequence General transcription factor Binding protein Nuclear Proteins DNA Cell Biology Molecular biology Introns Globins DNA binding site A-site Oligodeoxyribonucleotides HeLa Cells Transcription Factors |
| Zdroj: | Journal of Biological Chemistry. 270:28448-28456 |
| ISSN: | 0021-9258 |
| Popis: | The second intron of the human beta globin gene (beta IVS2) has been previously identified as a region required for proper expression of beta globin. To further characterize this region, we have footprinted the entire beta IVS2 and have analyzed regions of interest by electrophoretic mobility shift assay. Through these studies we have identified four utilized binding sites for the erythroid regulatory factor GATA-1, two sites bound by general transcription factor Oct-1, two sites bound by the nuclear matrix attachment DNA binding protein special A-T-rich binding protein 1, and a site bound by a potential homeobox protein. Additionally, we have found several factors displaying temporal or tissue specificity by electrophoretic mobility shift assay, which may be potentially involved in the regulation of beta globin expression. These proteins are not supershifted by antibodies to factors important in erythroid regulation such as GATA-1, NFE-2, or YY1, or by antibodies against more general transcription factors. |
| Databáze: | OpenAIRE |
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