Rugged and accurate quantitation of colchicine in human plasma to support colchicine poisoning monitoring by using turbulent-flow LC-MS/MS analysis
| Autor: | Colette Latour, Olivier Laprévote, M. Soichot, E. Bourgogne |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Electrospray
Analyte Chromatography Chemistry Calibration curve Elution Clinical Biochemistry General Medicine Zinc Sulfate Analytical Chemistry Medical Laboratory Technology chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry Calibration Colchicine Protein precipitation Chemical Precipitation Humans Sample preparation Centrifugation General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid |
| Zdroj: | Bioanalysis. 5(23) |
| ISSN: | 1757-6199 |
| Popis: | Background: Colchicine is a common drug used in inflammatory diseases. The narrow therapeutic index requires fast and reliable techniques for its quantitation. An online, automated sample preparation using TurboFlow™ technology combined with triple-stage quadrupole MS detection was applied to identify colchicine in human plasma and follow intoxications. Methodology: Plasma samples (200 µl) were mixed with deuterated colchicine and protein precipitation ZnSO4 solutions. After centrifugation, supernatants were extracted onto a Cyclone P TurboFlow column and eluted onto a narrowbore Hypersil™ GOLD column with a methanol/water gradient. Analytes were monitored in SRM mode (positive electrospray). Results: Total run time was 9.5 min. Calibration curves ranged from 0.342 to 17.1 ng/ml, with significant linearity (R2 >0.99). Inter- and intra-assay precisions were |
| Databáze: | OpenAIRE |
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