Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats
| Autor: | Gaïa Lépine, Jean-François Huneau, Didier Rémond, Véronique Mathé, Jérémie David, Dominique Hermier, Laetitia Guérin-Deremaux, Catherine Lefranc-Millot, Nathalie Poupin, François Mariotti, Sergio Polakof, Hélène Fouillet |
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| Přispěvatelé: | Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Physiologie de la Nutrition et du Comportement Alimentaire (PNCA (UMR 0914)), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Roquette Frères, Métabolisme et Xénobiotiques (ToxAlim-MeX), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This study was supported by the French National Research Institute for Agriculture, Food and Environment (INRAE) for the experimental and analytical costs (Action Prioritaire ProVegOmics). |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
protein synthesis/turnover
Nutrition and Dietetics de novo lipogenesis metabolic tracer protein and amino acid metabolism Medicine (miscellaneous) [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism natural isotopic abundance [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Journal of Nutrition Journal of Nutrition, 2022, 153 (3), pp.645-656. ⟨10.1016/j.tjnut.2022.12.029⟩ |
| ISSN: | 0022-3166 |
| Popis: | International audience; Background: Plant proteins (PP) have been associated with better cardiovascular health than animal proteins (AP) in epidemiological studies. However, the underlying metabolic mechanisms remain mostly unknown. Objective: Using a combination of cutting-edge isotopic methods, we aimed at better characterizing the differences in protein and energy metabolisms induced by dietary protein sources (PP vs AP), in a prudent or western dietary context.Methods: Male Wistar rats (n=44, 8 weeks old) were fed for 4.5 months with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat), and being normal (NSF) or high (HFS) in sucrose (6 or 15%kcal, respectively) and saturated fat (7 or 20%kcal, respectively). We measured body weight and composition, hepatic enzyme activities and lipid content, and plasma metabolites. In the intestine, liver, adipose tissues and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis (DNL) using a 2H labelling method. Data were analyzed using ANOVA and mixed models.Results: At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P |
| Databáze: | OpenAIRE |
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