Functional Coiled-Coil-like Assembly by Knob-into-Hole Packing of Single Heptad Repeat
| Autor: | Roy Beck, Guy Jacoby, Linda J. W. Shimon, Ehud Gazit, Vasantha Basavalingappa, Sudipta Mondal |
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| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular Protein Folding Materials science Protein Conformation Amino Acid Motifs Nanofibers Supramolecular chemistry General Physics and Astronomy Biocompatible Materials Sequence (biology) 02 engineering and technology Crystallography X-Ray 010402 general chemistry 01 natural sciences Protein Structure Secondary Side chain General Materials Science Coiled coil General Engineering 021001 nanoscience & nanotechnology Liquid Crystals 0104 chemical sciences Folding (chemistry) Heptad repeat Crystallography Lyotropic liquid crystal Sequence space (evolution) Protein Multimerization Peptides 0210 nano-technology |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.9b04148 |
| Popis: | Coiled-coil peptides represent the principal building blocks for structure-based design of bionanomaterials. The sequence-structure relationship and precise nanoscale ordering of the coiled-coil helices originate from the knob-into-hole (KIH) packing of side chains. The helical interface stabilized by the KIH interaction is known to have chain lengths ranging from 30 to 1000 residues. Yet the shortest peptide required for oligomerization through KIH assembly is still unknown. Here, we report that through atomic resolution a minimal seven-residue amphipathic helix forms a different type of KIH motif, termed "supramolecular KIH packing", which confers an exceptional stability to the helical dimers. Significantly, at a low pH, the peptide self-assembles into nanofibers with coiled-coil architecture resembling the natural fibrous proteins. Furthermore, hierarchical ordering of the nanofibers affords lyotropic liquid crystals composed of a shortest natural helical sequence. Thus, this study expands the sequence space for a coiled-coil folding manifold and provides another paradigm for designer nanomaterials from minimal helical sequences. |
| Databáze: | OpenAIRE |
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