PC13 embryonal carcinoma cells produce a heparin-binding growth factor
| Autor: | J.H. van Veggel, S.W. de Laat, E.J.J. van Zoelen, T. M. J. Van Oostwaard |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.medical_specialty
Ratón medicine.medical_treatment Basic fibroblast growth factor Biology Fibroblast growth factor Chromatography Affinity Cell Line Embryonal carcinoma Mice chemistry.chemical_compound Internal medicine medicine Animals Secretion Growth Substances Cells Cultured Chromatography High Pressure Liquid Heparin Heparin Binding Growth Factor Growth factor Teratoma Cell Biology medicine.disease Molecular biology Endocrinology chemistry Cell culture Fibroblast Growth Factor 2 Mitogens |
| Zdroj: | Experimental Cell Research. 169:280-286 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(87)90190-x |
| Popis: | A polypeptide growth factor has been isolated from serum-free medium conditioned by mouse PC13 embryonal carcinoma cells, which is strongly mitogenic for Swiss 3T3 fibroblasts. On a CM-2-SW high-performance liquid chromatography cation-exchange column at low pH, this growth factor elutes at a salt concentration very close to that of basic fibroblast growth factor (FGF). The growth factor is mitogenic for a mesodermal derivative of embryonal carcinoma (EC) cells, but not for differentiated derivatives with endodermal or ectodermal characteristics, again similar to FGF. The PC13-derived growth factor binds to heparin-Sepharose, and elutes from this column at similar salt concentrations as FGF. These data demonstrate that PC13 embryonal carcinoma cells produce a basic heparin-binding growth factor (HBGF). Since the initial purification steps are similar to those used by Heath & Isacke (EMBO j 3 (1984) 2957 [7]) for isolation of a PC13 embryonal carcinoma-derived growth factor (ECDGF), which is cationic with a molecular weight (MW) close to that of FGF, the present heparin-binding growth factor (HBGF) is most likely identical with ECDGF. |
| Databáze: | OpenAIRE |
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