Autocatalytic cooperativity and self-regulation of ATPase pumps in membrane active transport
| Autor: | Weissmüller G, Bisch Pm |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adenosine Triphosphatases
Binding Sites biology Chemistry Stereochemistry ATPase Cell Membrane Biophysics Kinetic scheme Biological Transport Active Substrate (chemistry) Cooperativity General Medicine Membrane transport Models Biological Catalysis Autocatalysis Kinetics Membrane biology.protein Mathematical Computing Flux (metabolism) |
| Zdroj: | European Biophysics Journal. 22 |
| ISSN: | 1432-1017 0175-7571 |
| DOI: | 10.1007/bf00205813 |
| Popis: | We investigate the effect of autocatalysis on the conformational changes of membrane pumps during active transport driven by ATP. The translocation process is described by means of an alternating access model. The usual kinetic scheme is extended by introducing autocatalytic steps and allowing for dynamic formation of enzyme complexes. The usual features of cooperative models are recovered, i.e., sigmoid shapes of flux versus concentration curves. We show also that two autocatalytic steps lead to a mechanism of inhibition by the substrate as experimentally observed for some ATPase pumps. In addition, when the formation of enzyme complexes is allowed, the model exhibits a multiple stationary states regime, which can be related to a self-regulation mechanism of the active transport in biological systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink