Determinants of the brain-specific expression of the rat aldolase C gene: ex vivo and in vivo analysis
| Autor: | Axel Kahn, Pascale Briand, Muriel Thomas, Iman Makeh, Henriette Skala |
|---|---|
| Přispěvatelé: | Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1993 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Transgene [SDV]Life Sciences [q-bio] Molecular Sequence Data CAAT box Mice Transgenic Biology Biochemistry PC12 Cells 050105 experimental psychology Gene Expression Regulation Enzymologic 03 medical and health sciences Mice Fructose-Bisphosphate Aldolase Gene expression Animals Deoxyribonuclease I 0501 psychology and cognitive sciences Binding site Promoter Regions Genetic Gene 030304 developmental biology Regulation of gene expression 0303 health sciences Reporter gene Binding Sites Base Sequence Aldolase C 05 social sciences Brain 3T3 Cells DNA Molecular biology Rats DNA-Binding Proteins Oligodeoxyribonucleotides Mutation |
| Zdroj: | European Journal of Biochemistry European Journal of Biochemistry, Wiley, 1993, 218 (1), pp.143-151 |
| ISSN: | 0014-2956 1432-1327 |
| Popis: | International audience; A 115-bp promoter fragment of the aldolase C gene is sufficient for conferring neural cell specificity on a reporter gene, in cultured PC12 cells and in transgenic mice. In vitro DNase I protection experiments detected two footprints on the promoter, termed boxes A/A', and B. The 5' A/A' box contains overlapping Sp1 and Krox20/Krox24 binding sites; it binds Sp1 in fibroblasts (box A') and a different complex in brain (box A). Any deletion or mutation of this box that impairs protein recognition also suppresses promoter activity. The replacement of box A/A' by a Sp1 consensus binding site results in the loss of the brain specificity of expression in transgenic mice. Further 3', box B is composed of a 5' direct repeat and a 3' GC box consisting of overlapping Sp1 and Krox20/Krox24 binding sites. Mutation of the direct repeat subregion appears to be more deleterious for the promoter activity than mutation of the G+C-rich subregion. |
| Databáze: | OpenAIRE |
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