Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

Autor: Yves Dessaux, Carlos G. Nieto-Peñalver, Camille Garcia, Thibaut Léger, Lucía I. C. de Figueroa, Teik Min Chong, Kok-Gan Chan, Jean-Michel Camadro, Elisa Violeta Bertini, Mariela Analía Torres, Kar-Wai Hong
Přispěvatelé: Planta Piloto de Procesos Industriales Microbiológicos [Tucumán] (PROIMI), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Jacques Monod (IJM (UMR_7592)), Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), JiangSu University, University of Malaya [Kuala Lumpur, Malaisie], Shantou University [Shantou, China], UCSI University, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), This work was supported by the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, PIP 2015 N°0946, PU-E22920160100012CO), Agencia Nacional de Promoción Científica y Tecnológica (PICT 2016 N° 0532, PICT 2016 N° 2013), and Secretaría de Ciencia, Arte e Innovación Tecnológica from the Universidad Nacional de Tucumán (PIUNT D609). Kok-Gan Chan thanks the financial support from the University of Malaya (FRGS grant no. FP022-2018A). Carlos Nieto-Peñalver thanks the support from Université Paris Diderot through the Alicia Moreau Chair., Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), University of Malaya = Universiti Malaya [Kuala Lumpur, Malaisie] (UM)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Genomics
Genomics, Elsevier, 2021, 113 (6), pp.4352-4360. ⟨10.1016/j.ygeno.2021.11.017⟩
Genomics, 2021, 113 (6), pp.4352-4360. ⟨10.1016/j.ygeno.2021.11.017⟩
ISSN: 0888-7543
1089-8646
DOI: 10.1016/j.ygeno.2021.11.017⟩
Popis: International audience; Agrobacterium tumefaciens is considered a prominent phytopathogen, though most isolates are nonpathogenic. Agrobacteria can inhabit plant tissues interacting with other microorganisms. Yeasts are likewise part of these communities. We analyzed the quorum sensing (QS) systems of A. tumefaciens strain 6N2, and its relevance for the interaction with the yeast Meyerozyma guilliermondii, both sugarcane endophytes. We show that strain 6N2 is nonpathogenic, produces OHC8-HSL, OHC10-HSL, OC12-HSL and OHC12-HSL as QS signals, and possesses a complex QS architecture, with one truncated, two complete systems, and three additional QS-signal receptors. A proteomic approach showed differences in QS-regulated proteins between pure (64 proteins) and dual (33 proteins) cultures. Seven proteins were consistently regulated by quorum sensing in pure and dual cultures. M. guilliermondii proteins influenced by QS activity were also evaluated. Several up- and down- regulated proteins differed depending on the bacterial QS. These results show the QS regulation in the bacteria-yeast interactions.; [Figure: see text].
Databáze: OpenAIRE
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